Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 3531-3545
DOI: https://doi.org/10.20546/ijcmas.2018.703.406


Effect of Hydrolysis Temperature on in vitro Bioaccessibility and Antioxidant Properties of Unicorn Leather Jacket (Aluterus monoceros) Skin Collagen Hydrolysates Following Simulated Gastro-Intestinal Digestion
Vinoth Kumar Lakshmanan1, Jeya Shakila Robinson2*, Jeyasekaran Geevaretnam2, Velayutham Padmanaban1 and Wenhui Wu3
1Department of Fish Processing Technology, Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Tuticorin 628 008, India
2Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Tuticorin 628 008, India
3Controller of Examinations, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Nagapattinam, India
*Corresponding author
Abstract:

The aim of the research was to prepare collagen hydrolysate (CH) from unicorn leatherjacket (Aluterus monoceros) fish skin using papain at different hydrolysis temperature viz. 50C (CH-5), 200C (CH-20), 250C (CH-25) and 500C (CH-50) and to investigate the antioxidant properties (DPPH free radical-scavenging activity, ferric reducing power assay, ferric chelating activity and hydroxyl radical scavenging activity) prior to and after in vitro gastrointestinal (pepsin–pancreatin) simulated digestion in order to assess the bioaccessibility and biofunctional activities. The DH was higher (4.27%) for CH-50 within 300 min of hydrolysis than other CH’s. On contrary, ferric reducing and ferric chelating properties were comparatively higher in CH-5 than other CH’s, corresponding to 33% and 0.32 absorbance units, respectively at 8 mg/mL protein concentration. DPPH free radical-scavenging and hydroxyl radical scavenging activities were more or less similar in all CH’s. Subsequent in vitro gastrointestinal simulated digestion studies showed that the bioaccessibility of CH-50 was higher (54.25) after gastric and pancreatic digestion, but CH-5 exhibited good antioxidative property expressing 90% activity. FTIR spectral analysis further confirmed it through expression of major shifts in amide A, I and II peaks and disappearance of aromatic ring peaks in CH-5. The study thus indicated that CH with good antioxidant activity shall be produced by hydrolysis the skin at 50C, rather than at 500C.


Keywords: Unicorn leatherjacket, Collagen hydrolysates, Hydrolysis temperature, Simulated gastro intestinal digestion, Bioaccessibility, Biofunctionality
Download this article as Download

How to cite this article:

Vinoth Kumar Lakshmanan, Jeya Shakila Robinson, Jeyasekaran Geevaretnam, Velayutham Padmanaban and Wenhui Wu. 2017. Effect of Hydrolysis Temperature on in vitro Bioaccessibility and Antioxidant Properties of Unicorn Leather Jacket (Aluterus monoceros) Skin Collagen Hydrolysates Following Simulated Gastro-Intestinal Digestion.Int.J.Curr.Microbiol.App.Sci. 7(3): 3531-3545. doi: https://doi.org/10.20546/ijcmas.2018.703.406