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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 3476-3483
DOI: https://doi.org/10.20546/ijcmas.2018.703.399


Assessment of Pod Coats for the Study of Antioxidant Prospective in Cowpea, Mungbean and Moth Bean
Sukriti Nehra, Sushila Singh and Savita Rani*
Department of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar, Haryana, India
*Corresponding author
Abstract:

Cereals and pulses are the rich sources of protein and energy and thus hold an important place in human diet mainly in developing countries. In present study, pod coats of locally grown pulse crops (cowpea, mungbean and mothbean) were tested for various phytochemical constituents and antioxidant activity. Pod coats of these pulse crops were extracted with three solvents (acetone, ethylacetate and chloroform) and tested for total phenols, flavonoids, tannins, tocopherols and DPPH free radical scavenging activity. Highest extract yield was observed in acetone extract of mothbean (4.45%) while lowest in chloroform extract of cowpea (2.99%). Total phenolics content ranged between 7.24 mg GAEg-1 (chloroform extract of cowpea) to 15.59 mg GAEg-1 (acetone extract of mothbean). Flavonoid content of pod coats of these pulses varied from 3.30 mg CAEg-1 (chloroform extract of cowpea) to 6.05 mg CAEg-1 (acetone extract of mothbean). Tocopherols content was highest in ethylacetate extract of mungbean (5.76 mg/g of extract) while lowest in chloroform extract of cowpea (3.41 mg/g of extract). Tannin content was highest in acetone extract of mothbean (1.86 mg TAEg-1) while lowest in chloroform extract of mungbean (0.71 mg TAEg-1). Antioxidant activity was determined by DPPH method. Acetone extract of mothbean exhibited highest DPPH free radical scavenging activity (74.21%) while it was lowest in chloroform extract of cowpea (54.95%) at a concentration of 1.0 mg/mL of the extract. The results of the present analytical study revealed that pod coat of these three pulse crops may be a valuable source of natural antioxidants and are potentially applicable in food and medicinal industry


Keywords: Antioxidant activity, Flavonoids, Phenols, Tocopherols and tannins
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How to cite this article:

Sukriti Nehra, Sushila Singh and Savita Rani. 2018. Assessment of Pod Coats for the Study of Antioxidant Prospective in Cowpea, Mungbean and Moth Bean.Int.J.Curr.Microbiol.App.Sci. 7(3): 3476-3483. doi: https://doi.org/10.20546/ijcmas.2018.703.399