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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 3447-3467
DOI: https://doi.org/10.20546/ijcmas.2018.703.397


Genetic Diversity of Diazotrophs Nodulating Pigeon Pea in Arid and Semi-Arid Zones of Haryana, India
Kuldeep Singh*, Subha Dhull and Rajesh Gera
Department of Microbiology, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, Haryana, India
*Corresponding author
Abstract:

The genetic diversity within forty nine rhizobial strains isolated from root nodules of pigeon pea crop grown in pots holding 5 kg soils collected from arid and semiarid zones of Haryana, India was investigated by using PCR-RFLP of the ribosomal operon [16S rRNA gene] and 16S rRNA gene partial sequence analysis. Genomic DNA of these rhizobial isolates was tested for nod C and nifH gene primers and hence authenticated as rhizobia. Out of 49 rhizobial isolates, only 35 isolates showed nifH gene amplification by using two different set of primers. All rhizobial isolates were also amplified with 16S rDNA gene using 27F and 1378R primers. The amplified product was subjected to RFLP analysis using MspI and HaeIII restriction enzymes. Dendrogram based on 16S rDNA profiles using ARDRA, showed 18 different biotypes at 80% similarity coefficient. Most prevalent biotype was 13th type, which prevails in all the four studied districts. Geographical origin and soil properties are defining feature for rhizobial diversity, which group them into separate genotypes. These results may be important in formulating rhizobial inoculants specific for pigeon pea under arid and semi-arid zones for agriculture development.


Keywords: Pigeon pea, Diversity, Rhizobia, nifH, Dendrogram, Biotypes
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How to cite this article:

Kuldeep Singh, Subha Dhull and Rajesh Gera. 2018. Genetic Diversity of Diazotrophs Nodulating Pigeon Pea in Arid and Semi-Arid Zones of Haryana, India.Int.J.Curr.Microbiol.App.Sci. 7(3): 3447-3467. doi: https://doi.org/10.20546/ijcmas.2018.703.397