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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 3329-3337
DOI: https://doi.org/10.20546/ijcmas.2018.703.384


Phytochemical Screening and Antibacterial Activity of Hibiscus rosa - sinensis Leaf Extracts
Jyoti V. Vastrad* and Shameembanu A. Byadgi
All India Coordinated Research Project on Clothing and Textiles, Main Agriculture Research Station, University of Agricultural Sciences, Dharwad – 580005, Karnataka, India
*Corresponding author
Abstract:

Plants have been known to be a reservoir of secondary metabolites which are being exploited as source of bioactive substance for various pharmacological purposes. In the present study, fresh leaf extracts of hibiscus were prepared using ethanol, methanol and distilled water. The extracts were assessed for the presence of various classes of phytochemicals by subjecting to different tests and total phenolic content of each extract was carried out using FCR method. Results revealed that all the extracts of hibiscus contained alkaloids, flavonoids and tannins. Saponins and terpenoids were absent in all the leaf extracts of hibiscus. Phenolic concentration of hibiscus was high in ethanol (425.437mg/g) than methanol and distilled water extracts. Bioassay results revealed that crude ethanol extract of hibiscus leaves exhibited very good antibacterial activity against S. aureus and E. coli compared to 10% and 5% extract concentrations. Further, the fabric samples treated with 10% extract exhibited antibacterial activity only against Staphylococcus aureus. However, antibacterial activity was absent in the samples treated with 5% hibiscus leaf extract. Thus, the presence of bioactive photochemicals could lend the antibacterial potency to the Hibiscus leaves and therefore, could be utilized not only for their high nutritional values but also as a medical plant.


Keywords: Antibacterial activity, Escherichia coli, Hibiscus rosa-sinensis, Phytochemicals, Staphylococcus aureus, Total phenolic content
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How to cite this article:

Jyoti V. Vastrad and Shameembanu A. Byadgi. 2018. Phytochemical Screening and Antibacterial Activity of Hibiscus rosa - sinensis Leaf Extracts.Int.J.Curr.Microbiol.App.Sci. 7(3): 3329-3337. doi: https://doi.org/10.20546/ijcmas.2018.703.384