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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 3268-3274
DOI: https://doi.org/10.20546/ijcmas.2018.703.377


Genetic Diversity Assessment in Pigeonpea Cultivars Using Microsatellite (SSR) Markers
Nagaraj Hullur1*, B.N. Radha2, B. Basavaraja1, B.C. Channakeshava3 and M. Byregowda4
1Department of Seed Science and Technology, College of Agriculture, Karekere, Hassan-573225, India
2Department of Seed Science and Technology, College of Sericulture, Chintamani, India
3Department of Seed Science and Technology, College of Agriculture, UAS, GKVK, Bengaluru, India
4AICRP on Pigeonpea, UAS, GKVK, Bengaluru, India
*Corresponding author
Abstract:

Genetic diversity among 20 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) analysed using twelve SSR markers. Although most of the cultivars indicated polymorphism for the studied primers, cultivars BRG-2, BRG 5, GRG 818, HY3C, ICP 8863, ICPL 87119 and JKM 189 could amplify specific unique amplicon at higher molecular weight (138-148 bp) when primer CCB-8 was used. Similarly cultivars AKT 08-02, CoRG 9701, GRG 818, JKM 189, JKM 206, TS 3R, TTB, WRG 173 and WRP 1 could amplify specific unique amplicon at higher molecular weight (155-180 bp) when CCB-9 primer was used. In case of primer PB-3, cultivars AKT 08-02, BRG 1, BRG 3, CoRG 9701, GRG 822, ICP 8863 could amplify specific unique amplicon at higher molecular weight (280-340 bp) and primer PB-8 could amplify cultivars BDN 2, BRG 1, BRG-2, BRG 3, BRG 4, JKM 189, JKM 209, TS 3R, TTB-7 at higher molecular weight (305-320bp). Among the twelve primers (CCB-10, CCB-3, CCB-6, CCB-7, CCB-8, CCB-9, PB-1, PB-3, PB-7, PB-8, PB-9, PB-10) used in the present study for genetic diversity assessment CCB-8, CCB-9, PB-3 and PB-8 can be useful in detecting the genetic structure of the plants rapidly and easily by PCR technique via marker aided selection in early stages of growth.


Keywords: Pigeonpea diversity, SSR, Microsatellite markers
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How to cite this article:

Nagaraj Hullur, B.N. Radha, B. Basavaraja, B.C. Channakeshava and Byregowda, M. 2018. Genetic Diversity Assessment in Pigeonpea Cultivars Using Microsatellite (SSR) Markers.Int.J.Curr.Microbiol.App.Sci. 7(3): 3268-3274. doi: https://doi.org/10.20546/ijcmas.2018.703.377