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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 816-823
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.094


Production of Short Chain Length Polyhydroxyalkanoates by Bacillus megaterium PHB29 from Starch Feed Stock
Balakrishna Pillai Aneesh, Jaya Kumar Arjun, Thulasi Kavitha and Kumarapillai Harikrishnan*
Environmental Biology Laboratory, Rajiv Gandhi Centre for Biotechnology, Poojappura   Thiruvananthapuram-14, Kerala, India
*Corresponding author
Abstract:

Polyhydroxyalkanoates (PHAs) are accumulated in bacteria as an energy reserve and have attracted great scientific interest as a biodegradable, biocompatible alternative to conventional plastics. The study is focused on polyhydroxybutyrate (PHB) accumulation by an environmental bacterial isolate Bacillus megaterium PHB29 using starch as carbon feedstock. PHB accumulation in the isolate was visualized by staining techniques using Sudan Black B and Nile Red dyes. The biomass production as well as PHA accumulation in PHB29 was tested with starch as carbon source at different temperatures and found yielding 3.07 g/L cell dry mass with 73.46% (w/w) PHA accumulation at an optimum temperature 34oC. The extracted polymer was subjected to 1H NMR analysis and the spectrum showed signals for a methine group (-CH-), a methylene group (-CH2-) and a methyl group (-CH3) proving  the polymer as poly-3-hydroxybutyrate (PHB). The ratio of starch to PHB conversion of this isolate was found to be better than other reported strains of Bacillus megaterium as well as other species among the genus Bacillus.


Keywords: Poly-3-hydroxybutyrate, Bacillus megaterium, Amylase,Starch,1H NMR.
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How to cite this article:

Balakrishna Pillai Aneesh, Jaya Kumar Arjun, Thulasi Kavitha and Kumarapillai Harikrishnan. 2016. Production of Short Chain Length Polyhydroxyalkanoates by Bacillus megaterium PHB29 from Starch Feed Stock.Int.J.Curr.Microbiol.App.Sci. 5(7): 816-823. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.094