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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 3216-3226
DOI: https://doi.org/10.20546/ijcmas.2018.703.372


Stress Induced Callus Initiation and Shoot Regeneration in Ovary Culture of Marigold (Tagetes spp)
Thaneshwari1*, C. Aswath2 and N. Chaithra3
1ICAR- Indian Agricultural Research Institute, New Delhi- 110 012, India
2Division of Floriculture and Medicinal Crops-ICAR- Indian Institute of Horticultural Research, Bengaluru, 560 089, Karnataka, India
3SRF, MSc (Agri) Plant Biotechnology, Division of Floriculture and Medicinal Crops-ICAR- Indian Institute of Horticultural Research, Bengaluru, 560 089, Karnataka, India
*Corresponding author
Abstract:

Marigold is an important ornamental plant grown as garden plant and for flower decoration. It has been recognized as a key source of carotenoids; exclusively used in Pharmaceutical industries and poultry feed additives. However, improvement of this valuable species is hampered by its high heterozygosity, but presently the greatest emphasis is on development of haploids through ovary culture, the efficient and time-saving technique in varietal improvement. As different factors affect the outcome in the ovary culture to produce haploids, thus present research was conducted to investigate effect of different stress (heat shockto flower buds, varying concentrations of sucrose in media and different durations of light-dark periods) on proliferation of ovary-derived callus, and its subsequent regeneration in marigold cultivar Arka Agni. Accordingly, pretreatment of flower buds at 45 °C for 2hr resulted in best callus induction and shoot differentiation, while Murashige and Skoog (MS) media supplemented with 4 % sucrose, 4.44 µM BAP and 4.52 µM 2, 4-D, incubated in dark for 4 weeks at 25 °C yielded highest callus induction (89.66 %). Further, sub culturing on MS media supplemented with 4% sucrose, 4.44 µM BAP and 1.07 µM NAA exposed to 16/8 hr photoperiod lead to highest shoot differentiation rate (23.35%). Subsequently, regenerated shoots rooted readily on hormone free MS media.


Keywords: Ovary culture, Gynogenesis, Plant regeneration, Stress treatments, Tagetes erecta, Haploids
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How to cite this article:

Thaneshwari, C. Aswath and Chaithra, N. 2018. Stress Induced Callus Initiation and Shoot Regeneration in Ovary Culture of Marigold (Tagetes spp).Int.J.Curr.Microbiol.App.Sci. 7(3): 3216-3226. doi: https://doi.org/10.20546/ijcmas.2018.703.372