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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 785-793
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.090


Bactericidal Application of Biosynthesized Silver Nanoparticles
Hala Ezzat Abou El-Hassayeb*
Department of Marine Microbiology, National Institute of Oceanography and Fisheries, Alexandria, Egypt
*Corresponding author
Abstract:

Silver bionanoparticles have been known to have inhibitory and bacterial effects. Apart from standardizing the best parameter for the synthesis of silver nanoparticles  involved in reduction of silver ion to silver nanoparticles. The involvement of nitrate reductases as reducing agent was confirmed by biochemical assay. The nitrate reductase activity got reduced from  0.9876 mmole/min/ml to 0.3233 mmole/min/ml after biofabrication of silver nanoparticles. In the present study, Pseudomonas aeruginosa ATCC 9027 has been used for the biosynthesis of silver nanoparticles (SNPs). It was found that aqueous Ag ions in solution when exposed to Pseudomonas aeruginosa ATCC 9027 get reduced, thereby leading to the formation of silver nanoparticles. The formation of silver   nanoparticles was confirmed by the change in colour of the culture filtrate from yellow to brown after the addition of silver nitrate. The morphology and uniformity of silver nanoparticles were investigated by UV–Vis spectrum, X-ray diffraction and scanning electron microscope (TEM). These biosynthesized silver nanoparticles were also evaluated for their antimicrobial activity against Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 2592 and Escherichia coli ATCC 8739. It was interesting to note that Pseudomonas aeruginosa ATCC 9027 that biosynthesized the silver nanoparticle was most affected by its antibacterial activity applications.


Keywords: Pseudomonas aeruginosa, Biosynthesis,Silver nanoparticles, Nitrate reductase, antimicrobial activity.
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How to cite this article:

Hala Ezzat Abou El-Hassayeb. 2016. Bactericidal Application of Biosynthesized Silver NanoparticlesInt.J.Curr.Microbiol.App.Sci. 5(7): 785-793. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.090