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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2774-2789
DOI: https://doi.org/10.20546/ijcmas.2018.703.320


Elucidation of Biochemical Mechanism Involved in Microbial Degradation of 1:1 Metal Complex Dye Containing Simulated Wastewater
D.K. Patel1, D.R. Tipre1, T.L. Patel1, A. Kadam2, S.A. Agarwal1 and S.R. Dave3*
1Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad 380009, Gujarat, India
2Dongguk University, Department of Life Sciences, South Korea
3XRF, Loyola Centre for R & D, Xavier college campus, Ahmedabad 380009, Gujarat, India
*Corresponding author
Abstract:

A developed bacterial consortium showed potential for decolourization of two simulated wastewaters containing 1:1 metal complex acid dyes namely, Acid blue 158 and Acid black 52, separately. Ability of decolourization was found up to 1300-1650 mg L-1 of cumulative dye concentration in a fed batch system along with faster decolourization rate in batch mode at 100 mg L-1 concentration. Chromium removal efficiency of consortium showed metal sorption on bacterial cells. Oxidoreductive enzymes that are laccase, lignin peroxidase, tyrosinase, azoreductase and NADH-DCIP reductase were involved in the degradation of dyes. UV-Vis spectra, HPLC and FTIR analysis confirmed the biodegradation of the dyes. H1NMR and GC-HRMS analysis supported the prediction of possible biodegraded metabolites produced, on the basis of which degradation pathways were proposed with possible degradation intermediates. Reduced phytotoxicity was observed after treatment for Cicer aeriatinum whereas S. bicolor showed induced growth. Similarly, reduced microbial toxicity was observed (15-37%) for S. aureus and P. aeruginosa, both.


Keywords: Decolourization, Toxicity, Consortium, Metal complex dyes
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How to cite this article:

Patel, D.K., D.R. Tipre, T.L. Patel, A. Kadam, S.A. Agarwal and Dave, S.R. 2018. Elucidation of Biochemical Mechanism Involved in Microbial Degradation of 1:1 Metal Complex Dye Containing Simulated Wastewater.Int.J.Curr.Microbiol.App.Sci. 7(3): 2774-2789. doi: https://doi.org/10.20546/ijcmas.2018.703.320