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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2663-2668
DOI: https://doi.org/10.20546/ijcmas.2018.703.307


Speciation of Candida Isolates from Clinical Samples by using Conventional and Chromagar Method
P.T. Rudrappa1, S.C. Chandrashekar2* and M.N. Sumana3
1Departmnet of Microbiology BMC&RI, Bangalore, Karnataka, India
2Department of Microbiology SSMC, Tumkur, Karnataka, India
3Department of Microbiology JSSMC, Mysore, Karnataka, India
*Corresponding author
Abstract:

Candida species have been reported from many countries worldwide and a significant morbidity & mortality in immunocompromised individuals and hospitalised patients. The Candida species are now among the four most common causes of hospital associated infections, catheter associated, urinary tract and blood stream infections. This was a cross sectional study in this the clinical samples received in the Microbiology laboratory for routine culture form the source. The study was conducted in the department of Microbiology JSSMC Mysore. A total of 75 isolates of Candia species recovered from urine, sputum, vaginal swab, ear swab and blood culture were used and identified by standard protocol. A total of 75 Candida species were collected from urine (72%), sputum (21%), swabs (2.6%) and Blood (1.3%) of patients. The goal of the study was to show there is an increase in the incidence of Candida non albicans. Majority of the isolates in our study were urine and throat samples, only few isolates were obtained from blood, vaginal swabs and ear swabs. So we obtained sensitivity and specificity of Chrom agar for C. albicans as 100%. As a result it can be concluded that the use of Chrom agar Candida is an easy reliable method for presumptive identification of most of the Candida species especially Candida albicans, Candida tropicalis, Candida krusie, Candida parapsilosis and Candida dublineinsis.


Keywords: Candida isolates, Speciation, Chromagar
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How to cite this article:

Rudrappa, P.T., S.C. Chandrashekar and Sumana, M.N. 2018. Speciation of Candida Isolates from Clinical Samples by using Conventional and Chromagar Method.Int.J.Curr.Microbiol.App.Sci. 7(3): 2663-2668. doi: https://doi.org/10.20546/ijcmas.2018.703.307