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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2551-2561
DOI: https://doi.org/10.20546/ijcmas.2018.703.295


DNA Marker Based Characterization of Wheat Genotypes for Terminal Heat Tolerance
Amarjeet Kumar, Swati, Narendra Kumar Singh, Anil Kumar and Jai Prakash Jaiswal
Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar-263145,Udham Singh Nagar, Uttarakhand, India
*Corresponding author
Abstract:

In India, wheat is cultivated on around 29.8 mha that produces 92.5 mt with an average productivity of 3.1 t/ha. But in recent scenario of climate change our wheat cultivars succumb to the grave problem of terminal heat stress leading to substantial reduction in its production and productivity. Sixteen genotypes of wheat were used for assessing the molecular diversity for terminal heat stress tolerance against 14 SSR markers linked to the trait of interest. Results revealed that amplified alleles ranged from185 bp to 230 bp. The maximum number of polymorphic bands was generated with primers Xgwm 484 and Xcfd 43 that resulted in 4 amplicons followed by primer Xgwm 268 that resulted in 3 amplicons. The SSR primer Xgwm 484 amplified 4 polymorphic amplicons varying from size 220bp to 230 bp. The marker Xcfd 43 detected 4 SSR alleles. The dendrogram classified sixteen genotypes into two broad groups, A and B. The two groups were generated at a similar coefficient of 0.55. Group A consisted of six genotypes and was further subdivided into three clusters. Group B consisted of ten genotypes which were further subdivided into three clusters (cluster IV, V and VI). The mean HSI, grain yield and relative reduction in grain yield under stress condition over timely sown condition was the basis of categorization of genotypes correspond to the molecular data. Genotypes, DBW90, WH1021, HD3059, JOB666, UP2843 and WH1124 have proved their suitability for late sown condition out of which the former three genotypes (DBW90, WH1021and HD3059) has already been released for late sown condition.


Keywords: DNA marker, Wheat genotypes, Terminal heat tolerance
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How to cite this article:

Amarjeet Kumar, Swati, Narendra Kumar Singh, Anil Kumar and Jai Prakash Jaiswal. 2018. DNA Marker Based Characterization of Wheat Genotypes for Terminal Heat Tolerance.Int.J.Curr.Microbiol.App.Sci. 7(3): 2551-2561. doi: https://doi.org/10.20546/ijcmas.2018.703.295