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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2543-2550
DOI: https://doi.org/10.20546/ijcmas.2018.703.294


Effects of Dietary Herbal Supplementation on CASA Based Sperm Motion Traits in Subfertile Buffalo Bulls (Bubalus bubalis)
Shivkumar1*, Ajeet Kumar2, M. Honparkhe2, A.K. Singh2 and P.S. Brar2
1Department of Veterinary Clinical Complex, Veterinary College Gadag - 582101, Karnataka, India
2Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab, India
*Corresponding author
Abstract:

The study was conducted to evaluate the combined effect of herbs on pre-freeze and post-thaw sperm motion traits in subfertile buffalo bulls. Three subfertile buffalo bulls which were maintained on the basal ration along with this, they were orally supplemented with Panax ginseng roots, Shilajit, Withania somnifera roots, Tribulus terrestris fruits, Turnera diffusa leaves; Ptychopetalum olacoides bark each @ 400 mg/100 kg body weight and Pausinystalia yohimbe bark @ 300 mg/100 kg body weight of bulls for 60 days of treatment phase. A total of 144 semen ejaculates (16 ejaculates/ bull/ treatment phase) were collected during pre-treatment, treatment and post-treatment phase (each phase of 60 days). Semen was extended with Tris egg yolk extender and freezing was carried out in a bio-freezer. Semen was evaluated for sperm motion traits in pre-freeze and post-thaw semen by Computer Assisted Sperm Analyser (CASA). Herbal supplementation significantly (P<0.05) increased the pre-freeze curvilinear velocity (VCL) during treatment phase and post-treatment phase, whereas VSL, STR and BCF were significantly (P<0.05) increased only during post-treatment phase. Moreover, herbal treatment significantly (P<0.05) improved the post-thaw individual motility during the treatment and post-treatment phase, whereas rapid progressive motility was significantly higher only during the treatment phase. It could be concluded that dietary supplementation of herbs in combination improved the sperm motion traits in subfertile buffalo bulls.


Keywords: Subfertile buffalo bulls, Herbs, Pre-freeze, Post-thaw, CASA, Sperm motion traits
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How to cite this article:

Shivkumar, Ajeet Kumar, M. Honparkhe, A.K. Singh and Brar, P.S. 2018. Effects of Dietary Herbal Supplementation on CASA Based Sperm Motion Traits in Subfertile Buffalo Bulls (Bubalus bubalis).Int.J.Curr.Microbiol.App.Sci. 7(3): 2543-2550. doi: https://doi.org/10.20546/ijcmas.2018.703.294