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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2508-2518
DOI: https://doi.org/10.20546/ijcmas.2018.703.290


Isolation of Microalgae for Biomass and Lipid Enhancement through Plackett-Burmann Design
Premjyoti C. Patil* and Bharati S. Meti
Department of Biotechnology, Basaveshwar Engineering College, Bagalkot, Karnataka-587102, India
*Corresponding author
Abstract:

The algae are one among the biomass energy source which can be promising. The microalgae are the diverse organisms which can inhabit in different climatic conditions and adapt themselves and perpetuate forming their own niche. There is need to improve the local flora for biomass and lipid content instead of culturing exotic species. Chlorella the green microalgae, was isolated from fresh water and was characterized through molecular studies using 18s rRNA homology. The study has revealed that isolated species belong to Chlorophytes (green microalgae). The isolated species was further subjected to media optimization studies through Plackett-Burman which has revealed Urea being the highest positive effect of (7.6833) among seven variables (NaNO3, CaCl2, MgSO4, K2HPO4, KH2PO4, Urea, 2-4,D) R2 value of 93.16% and p value of 0.033(at 5 %) and K2HPO4 had a negative effect (-0.3833) for Biomass. NaNO3 had highest positive effect and K2HPO4 had least effect on the lipid content and R2 value of 61.14% and p value of 0.5788 (at 5 %) indicating the variables had less influence compared to Biomass. Further the variables which had highest positive effect on biomass and lipid will be selected for Response Surface methodology studies, aim will be to enhance both components in a single media, which will be the future scope of work.


Keywords: Chlorella, 18s rRNA, optimization, Plackett-Burman, Biomass, Algal lipid
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How to cite this article:

Premjyoti C. Patil and Bharati S. Meti. 2018. Isolation of Microalgae for Biomass and Lipid Enhancement through Plackett-Burmann Design.Int.J.Curr.Microbiol.App.Sci. 7(3): 2508-2518. doi: https://doi.org/10.20546/ijcmas.2018.703.290