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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2442-2450
DOI: https://doi.org/10.20546/ijcmas.2018.703.285


Root Cultures: In vitro Conservative Method for Metabolite Extraction from A. paniculata
Arpita Mahobia* and Zenu Jha
Department of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.) 492012, India
*Corresponding author
Abstract:

Medicinal plants are a significant part of medicine systems due to pharmaceutically important secondary metabolites produced. Wild collection is the source to fulfil the increasing demand of these compounds which make plant species vulnerable. So, conservation of species is very essential along with the harvesting of medicinal plants. Here, root cultures of medicinal plants under in vitro condition may provide a source of secondary metabolite without affecting the natural population. The root cultures accompanied with the agroinfection with Agrobacterium rhizogenes may provide a cost effective way to produce roots for secondary metabolite production. A. paniculata is a source of andrographolide which have several medicinal properties. This research targeted towards the development of a conservative process for commercial production of andrographolide with the development of hairy root cultures in large scale and elicitation of andrographolide along with the isolation of pure andrographolide from hairy root cultures. The results of the work included highest 62.83+1.69% hairy root induction percentage in 10.2 induction days when apical meristem co-cultivated with Agrobacterium rhizogenes (MTCC 532) in half strength MS supplemented with acetosyringone 400μM. The best media for mass multiplication was found to be semi solid media with 40.8 fold increase in total biomass yield (fresh weight) after six weeks of culturing. Among different elicitation treatments given, highest andrographolide content (4695.9 µg/gm DW) with 3.0 fold increase compared to control was obtained when hairy root cultures were exposed to 500C temperature for one hour. This technique will give biomass yield of 6.81 gm/L (dry weight) which will produce 32000 μg/6.81 gm DW andrographolide. The isolation of pure andrographolide crystals from hairy root cultures have been done which can be used further for screening and utilization in pharmaceutical industries after screening in animal models.


Keywords: Root cultures, A. paniculata, Andrographolide, Hairy roots, etc
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How to cite this article:

Arpita Mahobia and Zenu Jha. 2018. Root Cultures: In vitro Conservative Method for Metabolite Extraction from A. paniculata.Int.J.Curr.Microbiol.App.Sci. 7(3): 2442-2450. doi: https://doi.org/10.20546/ijcmas.2018.703.285