Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 725-732
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.083


Association of Genetic Polymorphisms in a Sample of Iraqi Patients with Type1 Diabetes mellitus
Nuha J. Kandala and Ruaa H. Abdul Ridha*
 Biotechnology Department‚ College of science‚ Baghdad University‚ Iraq
*Corresponding author
Abstract:

Type 1diabetes (T1D) is an organ-specific autoimmunity characterized by the invasion of auto-reactive T lymphocytes to the cells of the Langerhans islets that secreted insulin and the production of autoantibodies versus pancreas antigens. In order to underline the role of the genes involved in this study‚ we investigate ‚using PCR-RFLP for CTLA-4gene‚ PCR-RFLP and sequencing for VDR gene. Polymorphisms of two single nucleotide polymorphisms (SNPs) belonging to both genes in 60 T1D patients and 30 healthy control from Iraqi population. The present local study demonstrated that VDR gene FoK-I‚ FF genotype (P = 0.003) ‚Ff genotype (P = 0.002) and F‚ f alleles (P = 0.002)  frequencies were significantly associated; VDR gene Bsm-IBB genotype (P=1.9×10-4)‚ bb genotype (P= 8.1×10-4) and B‚ b alleles (P=4.6×10-6) was significantly associated .CTLA-4-1722(T>C) was non significant association while +49 (A>G) GG genotype (P=0.036) and AA genotype (P=0.004) shows significant association. These result suggest the involvement of  VDR and CTLA-4gene in the genetic  susceptibility to T1D. Interestingly FoK-I‚ Bsm-I and +49 (A>G) contributes to increasing the risk to the disease in our population. However‚ Further studies are require to confirmed this finding especially the VDR gene andCTLA-4-1722(T>C)  gene investigated for the first time in Iraqi population.


Keywords: Type1 Diabetes‚VDR gene‚CTLA-4 gene‚ polymorphisms.
Download this article as Download

How to cite this article:

Nuha J. Kandala and Ruaa H. AbdulRidha. 2016. Association of Genetic Polymorphisms in a Sample of Iraqi Patients with Type1 Diabetes mellitus.Int.J.Curr.Microbiol.App.Sci. 5(7): 725-732. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.083