Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2313-2321
DOI: https://doi.org/10.20546/ijcmas.2018.703.271


Early Screening of Fusarium Wilt and Molecular Analysis of Banana Variants
H. Kishor1*, G. Prabhuling2, Y.C. Abhijith3, N. Manjunatha4 and Y.P. Prasad5
1Aeroponics Centre, PRS Horticulture Farm, Chikkaballapura, Karnataka-562101, India
2Directorate of Research, University of Horticultural Sciences, Bagalkot, Karnataka, India
3Department of Fruit Science, College of Horticulture, GKVK campus, Bengaluru, India
4Department of Fruit Science, College of Horticulture, Kolar, India
5Department of Plant Pathology, University of Agricultural and Horticultural Sciences, Shimoga, India
*Corresponding author
Abstract:

Banana and plantains is the most important tropical fruit crop in the world, which serves as a major food and source of income in many countries. Bananas are severely infected by several pathogens one such Fusarium oxysoporum f. sp. Cubense which causes significant crop losses in the fields. In this study fusarium wilt screening was carried out on chemically induced mutants and molecular analysis was done to assess the acquired resistance to Fusarium oxysoporum f. sp. cubense. From the early screening we observed that Ethyl Methane Sulphonate derived mutants are more sensitive which ranged from 9.5-11.4%. Whereas, several mutants obtained from Sodium Azide were resistant and was comparable with resistant cv. Poovan. Similar correlation analysis with molecular markers also revealed comparable results with the early screening. RAPD primers (OPN-06 and OPR-07) showed resistance specific bands and were used for the testing variation among mutants, after confirming its reproducibility. Putative resistant and susceptible mutants along with Poovan banana when amplified with OPN 06 showed the presence of a major band at 1500 bp and 200 bp which evidenced the occurrence of random mutations in genome.


Keywords: Mutation, FOC, Screening, Molecular analysis
Download this article as Download

How to cite this article:

Kishor, H., G. Prabhuling, Y.C. Abhijith, N. Manjunatha and Prasad, Y.P. 2018. Early Screening of Fusarium Wilt and Molecular Analysis of Banana Variants.Int.J.Curr.Microbiol.App.Sci. 7(3): 2313-2321. doi: https://doi.org/10.20546/ijcmas.2018.703.271