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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2165-2169
DOI: https://doi.org/10.20546/ijcmas.2018.703.255


Genotypic Studies of Carbapenem Resistant Acinetobacter baumanii (CRAB) with Special Reference to Presence of OXA bla Genes
Anahita Bhesania Hodiwala*, Amrita Talukdar and Revati Sharma
Department of Microbiology, MGM Medical College and Hospital, Navi Mumbai, India
*Corresponding author
Abstract:

Acinetobacter baumannii is a significant pathogen in health care settings, where it causes a multitude of infections that include bacteremia, pneumonia, meningitis and urinary tract and wound infections. The increasing importance of infections caused by Acinetobacter spp., and the multi-drug resistance of the strains render studies of epidemiology and antibiotic resistance necessary for the prevention of further infections with this organism. Hence the study was carried out with the following aim and objective - to screen the Carbapenem Resistant Acinetobacter baumanii (CRAB) isolates for the presence of blaOXA-23, blaOXA-24, blaOXA-51, blaOXA-58-like gene by PCR. Polymerase chain reaction (PCR) was performed for the detection of OXA (blaOXA 23 like, blaOXA 24 like, blaOXA-51 like and blaOXA-58 like genes). Out of 190, 63% (120/190) were CRAB strains of which (63/120) i.e. 53 % were found to be MBL producers by MHT. Among 120 A. baumannii, OXA genes were detected in 102 isolates. blaOXA 51 like (n = 100) and blaOXA -23 like (n = 98) were the most common and they coexisted in 96 isolates. blaOXA 24 and blaOXA 58 were not detected in any of the isolates. This study concludes that there is high incidence of Multi drug resistance seen in Acinetobacer baumanii (AB) isolates acquired from samples of hospitalized patients. blaOXA-23 like and blaOXA 51 like genes are the most common types of OXA carbapenamases contributing to carbapenem resistance in clinical isolates of A. baumannii. The co-production of OXA and metallo- betalactamases is not an uncommon phenomenon in A. baumannii. This will go a long way in early detection and also help control infections due to this highly resistant organism.


Keywords: Carbapenem Resistant Acinetobacter baumanii (CRAB), Acinetobacter baumanii (AB), OXA bla genes
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How to cite this article:

Anahita Bhesania Hodiwala, Amrita Talukdar and Revati Sharma. 2018. Genotypic Studies of Carbapenem Resistant Acinetobacter baumanii (CRAB) with Special Reference to Presence of OXA bla Genes.Int.J.Curr.Microbiol.App.Sci. 7(3): 2165-2169. doi: https://doi.org/10.20546/ijcmas.2018.703.255