National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The drug resistance of Plasmodium falciparum to different antimalarials, even recently to artemisinin derivatives, complicates the fight against malaria. This disease remains the leading cause of consultation in the health structures in Ivory Coast. It is therefore urgent to identify new molecules from the traditional pharmacopoeia that is very popular in Africa and recommended by WHO. The general objective of this study is the valorization of the Ivorian pharmacopeia by in vitro/ ex vivo evaluation of the antiplasmodial activity and carry out a phytochemical screening of crude extracts. We tested 6 crude extracts including 3 aqueous and 3 ethanolic on clinical isolates on the one hand and reference strains of chloroquine-sensitive P. falciparum (NF54) and chloroquine-resistant (K1) on the one hand. And we have investigated phytochemical screening by standard protocols. The inhibition of erythrocyte schizogony was determined by measuring the fluorescence of Sybr green intercalated in the parasite DNA, using a spectrofluorimeter. Then the IC50 values were determined by the online software ICEstimator antimalarial version 1.2. All the aqueous and ethanolic extracts of the three plants inhibit the growth of the parasite (Plasmodium falciparum). The aqueous extracts of Entandrophragma angolense (Is: 1,66±0,16 μg / mL ;NF54 : 3,85μg / mL et K1 : 6μg / mL) and Griffonia simplicifolia (Is: 1,48±0,08μg / mL ; NF54 :1,91μg / mL et K1 : 12,66μg / mL) have the best antimalarial activity. The different extracts are rich in alkaloid, sterols and polyterpenes, saponin then polyphenols and flavonoid. The results therefore justify the traditional use of the plants in the treatment of malaria in the Department of Agboville (Ivory Coast).
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