Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2088-2095
DOI: https://doi.org/10.20546/ijcmas.2018.703.245


In Vitro/ Ex Vivo Antiplasmodial Activity and Phytochemical Screening of Crude Extracts Entandrophragma angolense (Welw.) C. DC., Griffonia simplicifolia (Vahl ex DC.) Baill .et Uapaca guineensis Müll. Arg. three Plants of Ivorian Pharmacopeia in the Treatment of Malaria
M.R. Offoumou1*, G.R. Kipre2, D.S. Kigbafori3,4, D. Camara1, A.J. Djaman2,5 and G.N. Zirihi1
1Université Félix Houphouët-Boigny, Laboratoire de Botanique, UFR Biosciences,
Abidjan, Côte d’Ivoire.
2Université Félix Houphouët Boigny, Laboratoire de Pharmacodynamie Biochimique, UFR Biosciences, Abidjan, Côte d’Ivoire.
3Université Félix Houphouët Boigny, Laboratoire de Zoologie et Biologie Animale, UFR Biosciences, Abidjan, Côte d’Ivoire.
4Centre Suisse de Recherches Scientifiques en Côte d'Ivoire (CSRS), Abidjan, Côte d’Ivoire
5Laboratoire de Biochimie, Institut Pasteur de Côte d’Ivoire
*Corresponding author
Abstract:

The drug resistance of Plasmodium falciparum to different antimalarials, even recently to artemisinin derivatives, complicates the fight against malaria. This disease remains the leading cause of consultation in the health structures in Ivory Coast. It is therefore urgent to identify new molecules from the traditional pharmacopoeia that is very popular in Africa and recommended by WHO. The general objective of this study is the valorization of the Ivorian pharmacopeia by in vitro/ ex vivo evaluation of the antiplasmodial activity and carry out a phytochemical screening of crude extracts. We tested 6 crude extracts including 3 aqueous and 3 ethanolic on clinical isolates on the one hand and reference strains of chloroquine-sensitive P. falciparum (NF54) and chloroquine-resistant (K1) on the one hand. And we have investigated phytochemical screening by standard protocols. The inhibition of erythrocyte schizogony was determined by measuring the fluorescence of Sybr green intercalated in the parasite DNA, using a spectrofluorimeter. Then the IC50 values were determined by the online software ICEstimator antimalarial version 1.2. All the aqueous and ethanolic extracts of the three plants inhibit the growth of the parasite (Plasmodium falciparum). The aqueous extracts of Entandrophragma angolense (Is: 1,66±0,16 μg / mL ;NF54 : 3,85μg / mL et K1 : 6μg / mL) and Griffonia simplicifolia (Is: 1,48±0,08μg / mL ; NF54 :1,91μg / mL et K1 : 12,66μg / mL) have the best antimalarial activity. The different extracts are rich in alkaloid, sterols and polyterpenes, saponin then polyphenols and flavonoid. The results therefore justify the traditional use of the plants in the treatment of malaria in the Department of Agboville (Ivory Coast).


Keywords: Medicinal plants, Antiplasmodial, Sybr green, Plasmodium falciparum, Agboville
Download this article as Download

How to cite this article:

Offoumou, M.R., G.R. Kipre, D.S. Kigbafori, D. Camara, A.J. Djaman and G.N. Zirihi. 2018. In Vitro/ Ex Vivo Antiplasmodial Activity and Phytochemical Screening of Crude Extracts Entandrophragma angolense (Welw.) C. DC., Griffonia simplicifolia (Vahl ex DC.) Baill .etUapaca guineensis Müll. Arg. Three Plants of Ivorian Pharmacopeia in the Treatment of Malaria.Int.J.Curr.Microbiol.App.Sci. 7(3): 2088-2095. doi: https://doi.org/10.20546/ijcmas.2018.703.245