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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2063-2073
DOI: https://doi.org/10.20546/ijcmas.2018.703.242


Ibuprofen Antifungal Activity on Both Planktonic and Biofilm Forms of Fluconazole-Resistant Candida spp. Strains and its Mechanism of Action Evaluated by Flow Cytometry
Letícia S. Sampaio1,2, Cecília R. da Silva1,3, Rosana S. Campos1,3, Francisca B.S.A. do Nascimento1,2, João B.A. Neto1,2, Lisandra J. da Silva1, Brenda S. Gaspar1, Helaine A. Queiroz1, Fátima D.D. Barroso1, Jacó R.L. Mesquita7, Manoel O. de Moraes4, Bruno C. Cavalcanti4, Hemerson I.F. Magalhães5, Iri S.P. Lima6 and Hélio V.N. Júnior1,2
1 Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN)
2Department of Pathology and Legal Medicine, School of Medicine
4Department of Physiology and Pharmacology, Neuropharmacology Laboratory
6Department Pharmacology, School of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil
3Christus University Center, Fortaleza, CE, Brazil
5School of Pharmacy, Federal University of Paraiba, João Pessoa, PB, Brazil;
7São José Hospital, Fortaleza, CE,Brazil
*Corresponding author
Abstract:

Over the past few decades, researches have reported an increased incidence of invasive candidiasis and suggested that the majority of infections produced by this pathogen are associated with biofilm growth, which are more resistant to antimicrobial agents than planktonic cells. In addition to these problems, there are few antifungal agents available and it is necessary the development of novel therapeutic strategies. In that context, this study aimed to assess the antifungal potential of ibuprofen against fluconazole-resistant Candida spp. planktonic and biofilm forms proposing a new view to the drug that are used with other therapeutic indications. After 24 h, the fluconazole-resistant Candida spp. strains showed minimum inhibitory concentration (MIC) in the ranges of 1.5–3 mg/mL for ibuprofen by the broth microdilution method (M27-A3). According to our data by flow cytometry, ibuprofen cause fungal death after damaging the plasma and mitochondrial membrane associated to an increase of phosphatidylserine externalization which activates apoptotic signaling pathways. Regarding biofilm-forming isolates, ibuprofen promotes a reduction of viability cell in mature biofilm of Candida spp. (p<0.05). Therefore, it was concluded that ibuprofen is capable of inhibit the growth in vitro of Candida spp. fluconazole-resistant, both in planktonic as biofilm form, inducing cell death by apoptosis.


Keywords: Candida spp., Resistance, Ibuprofen, Biofilm, Apoptosis
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How to cite this article:

Letícia S. Sampaio, Cecília R. da Silva, Rosana S. Campos, Francisca B.S.A. do Nascimento, João B.A. Neto, Lisandra J. da Silva Brenda S Gaspar Helaine A. Queiroz Fátima D.D. Barroso Jacó R.L. Mesquita, Manoel O. de Moraes, Bruno C. Cavalcanti, Hemerson I.F. Magalhães, Iri S.P. Lima and Hélio V.N. Júnior. 2018. Ibuprofen Antifungal Activity on Both Planktonic and Biofilm Forms of Fluconazole-Resistant Candida spp. Strains and its Mechanism of Action Evaluated by Flow Cytometry.Int.J.Curr.Microbiol.App.Sci. 7(3): 2063-2073. doi: https://doi.org/10.20546/ijcmas.2018.703.242