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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 1910-1926
DOI: https://doi.org/10.20546/ijcmas.2018.703.227


Cold Active Lipases Produced by Cryptococcus sp. Y-32 and Rhodococcus erythropolis N149 Isolated from Nella Lake, Antarctica
Abhas Kumar Maharana* and Shiv Mohan Singh
Polar Biology Laboratory, National Centre for Antarctic and Ocean Research, Vasco-da Gama, Goa-403804, India
*Corresponding author
Abstract:

Cold active lipase was investigated by bacteria and yeast isolates from the core sample of Nella Lake, Larsemann Hills region, East Antarctica. Among potential yeasts and bacteria producing lipases, best isolates were identified as Cryptococcus sp. Y-32 and Rhodococcus erythropolis N149 by molecular technique. In order to enhance the lipase production capabilities, both the isolate again subjected for optimization processes using various physiological (temperature and pH) and chemical (Carbon, nitrogen, minerals and various substrates like oils and triglycerides) parameters. The results indicated that a supplement of 1% w/v fructose, 0.1% w/v KCl and 2% v/v tween 80 at pH 8.5 and 15°C enhances the lipase production by 9.81-fold (6.81 U/ml) using Rhodococcus erythropolis N149. In other hands, the activators are 1% w/v of galactose and peptone, 0.1% w/v KCl and 2.5% v/v ghee at pH 11.5 and 15°C enhances the lipase production by 4.01-fold (3.35 U/ml) using Cryptococcus sp. Y-32. The present study successfully produced cold-active lipases with novel properties like low temperature and high pH stability, which can be used in the degradation of lipid wastes in cold regions and also can be used for detergent formulation for cold temperature washing of delicate clothes.


Keywords: Cold active, Larsemann Hills, Lipase, Psychrotolerant, Sediment core
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How to cite this article:

Abhas Kumar Maharana and Shiv Mohan Singh. 2018. Cold Active Lipases Produced by Cryptococcus sp. Y-32 and Rhodococcus erythropolis N149 Isolated from Nella Lake, Antarctica.Int.J.Curr.Microbiol.App.Sci. 7(3): 1910-1926. doi: https://doi.org/10.20546/ijcmas.2018.703.227