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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 1843-1847
DOI: https://doi.org/10.20546/ijcmas.2018.703.217


Antibiotic Resistance in Pseudomonas aeruginosa Strains Isolated from Various Clinical Specimens
K. Shirisha and R. Shyamala*
Department of Microbiology, Malla Reddy Medical College for Women, Hyderabad, Telangana, India
*Corresponding author
Abstract:

Pseudomonas aeruginosa is primarily an opportunistic pathogen that causes infections in hospitalized patients particularly in burn patients where the skin host defences are destroyed, orthopaedic related infections, respiratory diseases, immunosuppressed and catheterised patients. Clinical samples i.e., pus, urine, blood, sputum, CSF, throat swab, pleural fluid were collected from inpatients and out patients attending SVS Hospital Mahabubnagar during the period of 1-6-2009 to 31-01-2010. The specimens were inoculated on Nutrient agar, Blood agar, and MacConkey agar and selective medium as cetrimide agar and incubated aerobically overnight at 37degree Centigrade for 24 hours and then examined next day for growth. Pseudomonas aeruginosa was isolated from the following samples: - 24 samples from pus (48%), 11 samples from urine (22%), 5 samples from blood (10%), 4 samples from sputum (8%), 3 samples from pleural fluid (6%), 2 samples from throat swab (4%) and 1 sample from CSF (2%). Isolation of Pseudomonas aeruginosa was highest in pus (48%). Maximum resistance (94%) was observed with piperacillin followed by amoxyclav and carbencillin. High resistance of Pseudomonas aeruginosa against various commonly used antibiotics showed the alarming situation. The control of drug resistant Pseudomonas aeruginosa required rational prescribing and proper use of antibiotics.


Keywords: Resistance, Pseudomonas aeruginosa, Samples
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How to cite this article:

Shirisha, K. and Shyamala, R. 2018. Antibiotic Resistance in Pseudomonas aeruginosa Strains Isolated from Various Clinical Specimens.Int.J.Curr.Microbiol.App.Sci. 7(3): 1843-1847. doi: https://doi.org/10.20546/ijcmas.2018.703.217