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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 1708-1717
DOI: https://doi.org/10.20546/ijcmas.2018.703.202


Assessment of Genetic Diversity among Twenty Indian Wheat (Triticum aestivum L.) Cultivars using Simple Sequence Repeat (SSR) Markers
Vandana Sharma1, Vaishali1*, Pushpendra Kumar1, Manoj Kumar Yadav1 and Pooran Chand2
1Department of Agriculture Biotechnology
2Department of Genetics and Plant Breeding, Sardar Vallabhbhai Patel University of Agriculture and Technology,
Meerut-250 110 (U.P), India
*Corresponding author
Abstract:

Bread wheat is most cultivated cereal used as food over 95% of the population. Twenty wheat genotypes were assayed to study the genetic diversity using molecular markers. The seventy-five alleles were identified with a mean of 2.34 alleles per locus using 32 SSR markers. Majority of SSR markers showed a high level of polymorphism. PIC values ranged from 0.05 (WMS-169) to 0.75 (CWM-107), with an average of 0.38 per primer. The RP value of primer ranges from 0.92 (WMC-177) to 1.94 (WMS-169) with an average value of 1.51, which explains the ability of primers to resolve the studied germplasm. According to similarity matrix, genetic similarity value ranged from 0.51 to 0.91. The lowest genetic similarity was observed between the WH711 and HD2733 genotypes and the maximum similarity was shown by genotype HD2864 with DBW71. Cluster analysis grouped the twenty wheat genotypes into two main clusters with one separate member. Results indicated that wheat cultivars had high genetic diversity that can be used in wheat breeding programs.


Keywords: Bread wheat, Genetic diversity, SSR, PIC, Resolving power
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How to cite this article:

Vandana Sharma, Vaishali, Pushpendra Kumar, Manoj Kumar Yadav and Pooran Chand. 2018. Assessment of Genetic Diversity among Twenty Indian Wheat (Triticum aestivum L.) Cultivars using Simple Sequence Repeat (SSR) Markers.Int.J.Curr.Microbiol.App.Sci. 7(3): 1708-1717. doi: https://doi.org/10.20546/ijcmas.2018.703.202