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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 1418-1429
DOI: https://doi.org/10.20546/ijcmas.2018.703.170


In situ Bioremediation of Chlorpyrifos by Klebsiella sp. Isolated from Pesticide Contaminated Agricultural Soil
Elizabeth Mary John, Edna Mary Varghese, N. Krishnasree and M.S. Jisha*
School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala - 686560, India
*Corresponding author
Abstract:

Chlorpyrifos (O,O-diethyl O-3, 5, 6-trichloro-2-pyridyl phosphorothioate) is a widely used broad-spectrum organophosphate pesticide. Widespread and indiscriminate use of Chlorpyrifos has led to severe environmental problems. Bioremediation would be the only eco-friendly solution for Chlorpyrifos persistence in the environment. Many bacteria are capable of degrading Chlorpyrifos in liquid media. Thus, the current study attempted to isolate Chlorpyrifos degrading bacteria from pesticide applied soil. The isolates were then screened using HPLC analysis to calculate the percentage of pesticide degradation. The isolate showing higher percentage of degradation was then morphologically and biochemically characterized. Molecular identification included 16S rRNA sequencing and phylogenetic analysis which revealed that the isolate showed close similarity to Klebsiella sp. In situ bioremediation study in soil was carried out using the above isolate. The total microbial count and the soil dehydrogenase activity in the bioaugmented treatment were found to be higher compared to the treatment containing soil + Chlorpyrifos. Thus, it can be inferred that the Klebsiella sp isolate was capable of degrading toxic Chlorpyrifos into non-toxic products which increased the growth of soil microorganisms and dehydrogenase activity.


Keywords: Organophosphate pesticides, Chlorpyrifos, Bioremediation, Bioaugmentation, Klebsiella sp.
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How to cite this article:

Elizabeth Mary John, Edna Mary Varghese, N. Krishnasree and Jisha, M.S. 2018. In situ Bioremediation of Chlorpyrifos by Klebsiella sp. Isolated from Pesticide Contaminated Agricultural Soil.Int.J.Curr.Microbiol.App.Sci. 7(3): 1418-1429. doi: https://doi.org/10.20546/ijcmas.2018.703.170