National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Chlorpyrifos (O,O-diethyl O-3, 5, 6-trichloro-2-pyridyl phosphorothioate) is a widely used broad-spectrum organophosphate pesticide. Widespread and indiscriminate use of Chlorpyrifos has led to severe environmental problems. Bioremediation would be the only eco-friendly solution for Chlorpyrifos persistence in the environment. Many bacteria are capable of degrading Chlorpyrifos in liquid media. Thus, the current study attempted to isolate Chlorpyrifos degrading bacteria from pesticide applied soil. The isolates were then screened using HPLC analysis to calculate the percentage of pesticide degradation. The isolate showing higher percentage of degradation was then morphologically and biochemically characterized. Molecular identification included 16S rRNA sequencing and phylogenetic analysis which revealed that the isolate showed close similarity to Klebsiella sp. In situ bioremediation study in soil was carried out using the above isolate. The total microbial count and the soil dehydrogenase activity in the bioaugmented treatment were found to be higher compared to the treatment containing soil + Chlorpyrifos. Thus, it can be inferred that the Klebsiella sp isolate was capable of degrading toxic Chlorpyrifos into non-toxic products which increased the growth of soil microorganisms and dehydrogenase activity.
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