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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:3, March, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 1098-1109
DOI: https://doi.org/10.20546/ijcmas.2018.703.131


Screening for Incidence of Microsporidian Parasite Enterocytozoon hepatopenaei (EHP) in Litopenaeus vannamei from Aquaculture Ponds in SPSR Nellore District of Andhra Pradesh, India
M. Raveendra1*, P. Hari Babu1, T. Neeraja1, D. Pamanna1, N. Madhavan1, A.S. Sahul Hameed2 and C.H. Srilatha3
1College of Fishery Science Muthukur, Nellore, Andhra Pradesh, India
2OIE Reference Laboratory for WTD, Department of Zoology, C. Abdul Hakeem College, Melvisharam, Tamil Nadu, India
3College of Veterinary Science, S.V.V.U Tirupati, India
*Corresponding author
Abstract:

Hepaotopancreatic Microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP), a microsporidian parasite to be associated with slow (retarded or stunted) growth and white feces syndrome (WFS) in cultured shrimp in many of the shrimp growing countries in Asia, also in India. In the present study, shrimp samples from various shrimp ponds from different Mandals of SPSR Nellore district, Andhra Pradesh, India, were collected over a period of five months (February 2016 to June 2016). Important diagnosis observed were histopathological studies, molecular technique (PCR). Histologically, the infected animals showed severe degeneration of hepaotopancreatic tubule, basophilic inclusions resembling the developmental stages of EHP were found in the epithelial cells and large number of spore aggregations was observed in the tubular lumen. Enlargement of haemal sinuses was also observed in some cases. From this study, out of 50 pond case studies, 31 cases were showing EHP symptoms with 62 % prevalence by using tools of detection like PCR and histology.


Keywords: EHP, HPM, Parasite, PCR, Shrimp, WFS

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How to cite this article:

Raveendra, M., P. Hari Babu, T. Neeraja, D. Pamanna, N. Madhavan, A.S. Sahul Hameed and Srilatha, C.H. 2018. Screening for Incidence of Microsporidian Parasite Enterocytozoon hepatopenaei (EHP) in Litopenaeus vannamei from Aquaculture Ponds in SPSR Nellore District of Andhra Pradesh, India.Int.J.Curr.Microbiol.App.Sci. 7(3): 1098-1109. doi: https://doi.org/10.20546/ijcmas.2018.703.131
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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