National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Sepsis in newborn infants has a high risk of morbidity and mortality. Despite advances in medicine, diagnosis of neonatal sepsis remains as a major challenge. The aim of this study is to unravel the role played by new network of biomarkers namely high mobility group box 1(HMGB1), thrombomodulin and heat shock protein (Hsp) 72 in addition to redox status in diagnosis of early onset neonatal sepsis before and after antibiotic therapy. The study enrolled 45 neonates, 30 of them had early onset sepsis (group 1), and 15 healthy neonates as a control group (group 2). Neonates with early onset sepsis were classified according to their culture status into culture-proven sepsis (n=19) (group 1a) and culture negative sepsis (n=11) (group 1b). Levels of HMGB1, thrombomodulin, Hsp72, nitric oxide (NO) and hydrogen peroxide (H2O2) were estimated. Studied biomarkers were investigated before and 5 days after antibiotic therapy. Serum levels of HMGB1, NO and H2O2 were significantly (p<0.05) higher in neonates with sepsis than in healthy neonates (65.7± 8.7 vs 38.2±9.9; 33.4±9.1 vs 13.6±1.8 and 59.6±7.9 vs 35.0±3.4 respectively) and in culture proven than culture negative neonates (70.4± 6.4 vs 57.5± 5.6; 36.4±8.8 vs 28.1±7.3 and 61.3±7.4 vs 55.3±7.0 respectively) before antibiotic therapy while thrombomodulin and Hsp72 were significantly (p<0.05) lower in neonates with sepsis than in healthy neonates (72.3±11.2 vs 144.2±15.6 and 14.6±1.6 vs 32.6±4.3 respectively) and in culture proven than culture negative neonates for thrombomodulin (67.8± 10.7 vs 80.1± 7.4) and non-significance for Hsp 72 showed p>0.05 (14.6±1.5 vs 14.6±1.9) before antibiotic therapy. Results of all the aforementioned biomarkers improved after antibiotic treatment in sepsis group. HMGB1, thrombomodulin and Hsp72 are considered promising biomarkers for early diagnosis of early onset neonatal sepsis.
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