National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Hospital acquired infections are one of the major causes of morbidity and mortality in hospitalized patients, leading to an enormous increase in the cost of hospital care and to the emergence of new health hazards for the community. The study aimed to determine the bacteriological profile of hospital acquired infections along with prevalence of multidrug resistance and extended spectrum beta lactamase (ESBL) enzymes amongst the isolates. A total of 180 isolates of various organisms were isolated from different clinical samples during a period of one year from January 2014 to December 2014. The antibiotic susceptibility testing of the isolates was done on Mueller Hinton agar using antibiotics from different classes which included beta lactams, aminoglycosides, macrolides and fluoroquinolones. Multidrug resistance was defined as resistance of the isolate to three or more classes of antibiotics. Extended spectrum beta lactamase detection was done in Gram negative isolates by the combined disc diffusion method. The isolates included Staphylococcus aureus (32.22%), Pseudomonas aeruginosa (20.56%), Escherichia coli (16.11%), Coagulase negative Staphylococcus (12.22%), Klebsiella pneumoniae (8.89%), Acinetobacter sp. (5.56%), Enterococcus sp. (2.78%) and Proteus mirabilis (1.67%). Out of these 180 isolates, 27 (15%) isolates were found to show multidrug resistance, Pseudomonas aeruginosa and Acinetobacter being the major multidrug resistant organisms. Out of the 95 Gram negative organisms, 39 were confirmed to be ESBL producers by phenotypic method. The study concluded that the hospital strains of microorganisms are becoming more and more resistant to the currently available antibiotics. So, the antibiotics should be used more judiciously keeping the higher antibiotics in reserve which can be a solution to multidrug resistance.
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