Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 962-970
DOI: https://doi.org/10.20546/ijcmas.2018.703.114


-Glucosidase, -Xylosidase and -L-Arabinofuranosidase Production by Mutant Trichoderma atroviride 102C1 in Different Lignocellulosic Biomass Sources
Jessica Caroline Araujo Silva1, Daniela Sales Alviano1, Celuta Sales Alviano1
Elba Pinto da Silva Bon2 and Rodrigo Pires do Nascimento3*
1Universidade Federal do Rio de Janeiro (UFRJ), Centro de Ciências da Saúde (CCS), Instituto de Microbiologia Prof. Paulo de Góes, Departamento de Microbiologia Geral, Avenida Carlos Chagas Filho, 373, Bloco I, Laboratório 055, Zip Code: 21941-902, Rio de Janeiro, RJ, Brazil
2Universidade Federal do Rio de Janeiro (UFRJ), Centro de Ciências Matemáticas e da Natureza (CCMN), Instituto de Química, Departamento de Bioquímica, Avenida Athos da Silveira Ramos, 149, Bloco A, sala 539, Zip Code: 21941-909, Rio de Janeiro, RJ, Brazil
3Universidade Federal do Rio de Janeiro (UFRJ), Centro de Tecnologia (CT), Escola de Química, Departamento de Engenharia Bioquímica, Avenida Athos da Silveira Ramos, 149, Bloco E, sala 108, Zip Code: 21941-909, Rio de Janeiro, RJ, Brazil
*Corresponding author
Abstract:

Fungi are microorganisms considered decomposers of the organic matter, by the action of several enzymes that can present biotechnological potential, emphasizing the holocelulases (endoglucanases, exoglucanases, β-glucosidases, endoxilanases, β-xylosidases, among others). Among holocellulolytic fungi described in literature, Penicillium, Aspergillus and Trichoderma species are the most important. This study aimed to evaluate the efficiency in production of holocellulolytic accessories enzymes (-glucosidase, -xylosidase and -L-arabinofuranosidase) by Trichoderma atroviride 102C1, a mutant strain obtained in our laboratory, using different lignocelluloses biomass as substrates. The mutant strain 102C1 was cultivated at 28oC in salt mineral solution supplemented with corn steep liquor as nitrogen source (1.26% w/v), and wheat bran, sorghum bagasse, sugarcane straw (in natura and pre-treated with steam explosion) or sugarcane bagasse (in natura and pre-treated with steam explosion), as carbon source (2.5% w/v), 200 rpm, for 5 days. The best results for (-glucosidase, -xylosidase and -L-arabinofuranosidase activities were observed in sorghum bagasse (13.09 U.ml-1), sugarcane straw pretreated (5.24 U.ml-1) and wheat bran (192.6 U.ml-1), respectively, between 3 and 5 days fermentation. These results suggest the use of different agro-industrial by products to obtain holocellulolytic accessories enzymes by the mutant fungi T. atroviride 102C1 and the possibility for biotechnology process.


Keywords: Accessory enzymes, Sugarcane bagasse, Sugarcane straw, Sorghum bagasse, Wheat bran
Download this article as Download

How to cite this article:

Jessica Caroline Araujo Silva, Daniela Sales Alviano, Celuta Sales Alviano, Elba Pinto da Silva Bon and Rodrigo Pires do Nascimento. 2018. -Glucosidase, -Xylosidase and -L-Arabinofuranosidase Production by Mutant Trichoderma atroviride 102C1 in Different Lignocellulosic Biomass Sources.Int.J.Curr.Microbiol.App.Sci. 7(3): 962-970. doi: https://doi.org/10.20546/ijcmas.2018.703.114