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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 599-608
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.067


Bacteriologic Profile and Antimicrobial Resistance of Blood Culture Isolates of Septicemic Patients from Various Intensive Care Units in a Teaching Tertiary Care Institute of Haryana, India
Jyoti Sangwan1*, Pratibha Mane1, Prakriti Vohra1, Sumit Lathwal2 and A.K. Malik1
1Department of Microbiology, SHKM GMC, Mewat, Haryana, India
2ADH, 12 CORPS,C/O 56 APO, India
*Corresponding author
Abstract:

The objective of the study was to describe the pattern of bacterial isolates from the blood cultures in intensive care units of our institute and to determine their antibiotic sensitivity, so that the study can provide guidelines for choosing an effective antibiotic therapy in cases of septicaemia. In this prospective study 462 blood samples collected from clinically suspected cases of septicemia received over a period of one year. The isolates were identified by standard biochemical tests and antimicrobial susceptibility testing determined by National Committee for Clinical Laboratory Standards (NCCLS) guidelines. Positive cultures were obtained in 266 (57.6%) cases. Among culture positive isolates, 63.1% were Gram-positive and 29.7% by Gram negative bacilli and remaining 7.1% by Candida spp. Among Gram-positive isolates, Staphylococcus aureus was the predominant isolate followed by CONS and Enterococcus faecalis. Pseudomonas spp. was the most common Gram negative isolate followed by Klebsiella, Enterobacter, Citrobacter, Salmonella typhi, Proteus, E.coli and Acinetobacter spp. Incidence of MRSA and ESBL was 48% and 18.6% respectively Percentage sensitivity of isolates to individual antibiotic was also noted. This study provides information on antibiotic sensitivity of blood isolates in our set up. It may be a useful guide for physicians for initiating empiric therapy and will help in formulation of antibiotic policy in this part of the country.


Keywords: Septicaemia, Bacteraemia, Antibiotic resistance,Blood culture
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How to cite this article:

Jyoti Sangwan, Pratibha Mane, Prakriti Vohra, Sumit Lathwal and A.K. Malik. 2016. Bacteriologic Profile and Antimicrobial Resistance of Blood Culture Isolates of Saptecemic Patients from Various Intensive Care Units in a Teaching Tertiary Care Institute of HaryanaInt.J.Curr.Microbiol.App.Sci. 5(7): 599-608. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.067