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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 931-941
DOI: https://doi.org/10.20546/ijcmas.2018.703.110


In vitro Cultivation of Vesicular- Arbuscular Mycorrhizal Fungi and its Biological Efficacy
A.D. Bholay*, Sophia Barbosa and Kirti Jadhav
Department of Microbiology, K.T.H.M. College, Nashik, SP Pune University, MS, India
*Corresponding author
Abstract:

Crop improvement is significantly enhanced by the application of beneficial microbes. Progress in understanding the biology of mycorrhizal fungi is hampered by the limited number of species that can be successfully propagated and studied in vitro using resting spores. VAM spores were isolated from the rhizosphere soil and were cultivated in-vitro using Cooke Rose Bengal medium. In pot culture experiment, the plants treated with VAM isolates exhibited enhancement in growth than the control ones. After using mycorrhizal inoculum, the root colonization of crop plants was greatly affected. VAM fungal inoculation had significant effect on growth parameters like plant height, weight, MD and root colonization. Since spores could successfully infect wheat, rice and maize roots, this method provides a way of starting a pure-line culture of VAM fungus allowing to study the intraspecific variation of the fungus and to observe the presence, location and different developmental stages of hyphae and new fungal organs at an excellent medium and with a high rate of success and reproducibility.


Keywords: Mycorrhiza, VAM, Mycorrhizal dependency, Hydroponics
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How to cite this article:

Bholay, A.D., Sophia Barbosa and Kirti Jadhav. 2018. In vitro Cultivation of Vesicular- Arbuscular Mycorrhizal Fungi and its Biological Efficacy.Int.J.Curr.Microbiol.App.Sci. 7(3): 931-941. doi: https://doi.org/10.20546/ijcmas.2018.703.110