National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Seed spices are most valuable crops having a good export potential to boost national economy. Among all seed spices, fennel has their prime importance in medicinal and nationwide market. This crop is highly variable and rich in molecular variability. Two DNA based molecular marker techniques viz., Random Amplified Polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were used to study the molecular diversity among 17 fennel genotypes. A total of 26 polymorphic primers (16 random and 10 ISSR) were used. Amplification of genomic DNA of 17 genotypes, using RAPD analysis, yielded 79 fragments, in which 58 (73.41%) were polymorphic. The 10 ISSR primers produced 59 bands across 17 genotypes, of which 51 (86.44%) were polymorphic. The similarity coefficient ranged from 0.34 to 0.76 and 0.36 to 0.87. Based on the similarity matrix data dendrogram were prepared using UPGMA method. Genotypes were also classified into groups and several subgroups, respectively. Principal Coordinate Analysis (PCA) confirmed the separation of fennel genotypes into groups comparable to those from UPGMA analysis. The high rate of polymorphic lines generated by RAPD and ISSR markers indicated that the method is efficient to analyze molecular diversity in fennel genotypes and that the molecular divergence can be used to establish consistent heterotic groups between fennel genotypes. Hence, molecular markers proud to be, superior in assessing differences among genetically very similar genotypes and efficiently utilized in plant breeding programme for improvement of crops.
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