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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 794-809
DOI: https://doi.org/10.20546/ijcmas.2018.703.093


Molecular Diversity Analysis in Fennel (Foeniculum vulgare Mill) Genotypes and its Implications for Conservation and Crop Breeding
Sharda Choudhary*, Radheshyam Sharma, R.S. Meena and Arvind Kumar Verma
ICAR-National Research Centre on Seed Spices, Ajmer 305-206 (Rajasthan), India
*Corresponding author
Abstract:

Seed spices are most valuable crops having a good export potential to boost national economy. Among all seed spices, fennel has their prime importance in medicinal and nationwide market. This crop is highly variable and rich in molecular variability. Two DNA based molecular marker techniques viz., Random Amplified Polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were used to study the molecular diversity among 17 fennel genotypes. A total of 26 polymorphic primers (16 random and 10 ISSR) were used. Amplification of genomic DNA of 17 genotypes, using RAPD analysis, yielded 79 fragments, in which 58 (73.41%) were polymorphic. The 10 ISSR primers produced 59 bands across 17 genotypes, of which 51 (86.44%) were polymorphic. The similarity coefficient ranged from 0.34 to 0.76 and 0.36 to 0.87. Based on the similarity matrix data dendrogram were prepared using UPGMA method. Genotypes were also classified into groups and several subgroups, respectively. Principal Coordinate Analysis (PCA) confirmed the separation of fennel genotypes into groups comparable to those from UPGMA analysis. The high rate of polymorphic lines generated by RAPD and ISSR markers indicated that the method is efficient to analyze molecular diversity in fennel genotypes and that the molecular divergence can be used to establish consistent heterotic groups between fennel genotypes. Hence, molecular markers proud to be, superior in assessing differences among genetically very similar genotypes and efficiently utilized in plant breeding programme for improvement of crops.


Keywords: Fennel, ISSR, Molecular diversity, Molecular marker, Polymorphism, RAPD
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How to cite this article:

Sharda Choudhary, Radheshyam Sharma, R.S. Meena and Arvind Kumar Verma. 2018. Molecular Diversity Analysis in Fennel (Foeniculum vulgare Mill) Genotypes and its Implications for Conservation and Crop Breeding.Int.J.Curr.Microbiol.App.Sci. 7(3): 794-809. doi: https://doi.org/10.20546/ijcmas.2018.703.093