National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Citrus peels are known for the abundant amounts of polyphenols present, which have been proven to possess antimicrobial activity. The objective of this project was to determine the phenolic content and antibacterial capacity of orange (Citrus reticulata var. Kinnow) peel extracts against pathogenic strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Peel powder of Orange was subjected to polyphenolic extraction using different solvents viz., petroleum ether, ethanol, acetone, and methanol. Pathogenic bacterial strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae were screened for the antibacterial activity of the extracts using disc diffusion technique. The total phenolic content of the extracts was determined by the method involving Folin-Ciocalteau reagent and gallic acid standards, and was expressed as mg GAE/ml extract. As compared to other solvent extracts, acetone extract possessed high phenolic content with 17.6 mg GAE/ml of extract. It was also noticed that acetone extract possessed comparatively higher antibacterial potential, and it was shown to inhibit all four pathogenic bacterial strains. The Minimum Inhibitory concentration (MIC) of 68.75 µg/ml of acetone extract was found to inhibit Klebsiella pneumoniae and Escherichia coli, with no significant difference. Maximum zone of inhibition at MIC of acetone was found to be 7.93±0.065 mm in case of K. pneumoniae and 7.75±0.12 mm in E. coli.
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