National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Recycle and reusing waste takes little effort on our part, for a big difference to the world. Wastes samples like cow dung slurry, kitchen waste slurry and de-oiled seed cake slurry were collected from different places aseptically and microbiological analysis of the samples was undertaken. A total of 30 bacteria isolates were identified on the basis of cultural, morphological and bio chemical characteristics by using PIBwin software. The bacterial isolates belong to the genera Bacillus, Paenibacillus, Virgibacillus, Streptococcus, Micrococcus and Enterobacter sp. All the bacterial isolates were screened for different enzymatic tests. Fluorescence tests were investigated which revealed that 33.3% bacterial isolates were methanogenic. Biogas production was done by using anaerobic conventional digester. Kitchen waste slurry showed maximum production of bio gas than other samples. Final biogas production was done by using methanogenic isolates from cow dung slurry and the isolate A4 & A8 showed maximum bio gas production. Isolate A4 was identified as Bacillus licheniformis. Further molecular identification of isolate A8 was done. It was identified to be Enterobacter cloacae subsp. dissolvens strain A8 having GenBank accession number KM034810.
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