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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(1): 686-698
DOI: http://dx.doi.org/10.20546/ijcmas.2016.501.069


Detection and Genotyping of Human Papilloma Virus (HPV) As a Cause of Recurrent Early Pregnancy Loss
Aya A. Hammad, Neveen A. Ashaat, Samah F. Darwish, I.I. Moustafa and A.S. Amin
Faculty of Women for Arts, Science and Education, Ain Shams University, Egypt
Biotechnology Research Unit, Animal Reproduction Research, Giza, Egypt
Department of Obstetrics & Gynecology, Faculty of Medicine, Ain Shams University, Egypt
*Corresponding author
Abstract:

Human papillomavirus (HPV) is the most common sexually transmitted infection (STI). HPV is so common that nearly all sexually active men and women get it at some point in their lives. The objective of the this study is to assess whether or not there is an association between HPV and unexplained recurrent early pregnancy loss (REPL). Genomic DNA was extracted from the collected paired placenta and urine samples and was submitted to PCR methods with the primers MY09/11 and nPCR with the pair of primers MY09/MY11 and GP5+/6+. HPV-positive samples were typed by multiplex PCR for four high-risk HPV (HR-HPV) (HPV-16, 18, 31and 51). Agreement between paired sample results was evaluated. The results indicated that, HPV infection was found as 16.6% and 26.7% in placental samples, 6.6% and 36.7% in urine samples by using PCR and nPCR or real-time PCR respectively. HPV-16 was the predominance HPV type in both sample (10%) followed by HPV-31(6.6%) in both samples and HPV- types 51 and 18 (3.3%) in both samples. There was 3.3% co infection (infection by more than one type of HPV) in placenta and urine samples by types 16 and 51. There was poor agreement of placenta and urine samples results in generic and a moderate agreement for type- specific detection of HPV. The results also showed a significantly difference between number of miscarriage in HPV positive (3.38±0.744) and HPV negative (2.95±0.385) placenta samples. In conclusion HPV infection of trophoblast may cause placental dysfunction and is associated with adverse pregnancy outcomes, including recurrent early pregnancy loss


Keywords: Human papillomavirus (HPV), Molecular diagnostics, Recurrent early pregnancy loss, Nested PCR
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How to cite this article:

Int.J.Curr.Microbiol.App.Sci. 5(1): 686-698. doi: http://dx.doi.org/10.20546/ijcmas.2016.501.069