National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Human papillomavirus (HPV) is the most common sexually transmitted infection (STI). HPV is so common that nearly all sexually active men and women get it at some point in their lives. The objective of the this study is to assess whether or not there is an association between HPV and unexplained recurrent early pregnancy loss (REPL). Genomic DNA was extracted from the collected paired placenta and urine samples and was submitted to PCR methods with the primers MY09/11 and nPCR with the pair of primers MY09/MY11 and GP5+/6+. HPV-positive samples were typed by multiplex PCR for four high-risk HPV (HR-HPV) (HPV-16, 18, 31and 51). Agreement between paired sample results was evaluated. The results indicated that, HPV infection was found as 16.6% and 26.7% in placental samples, 6.6% and 36.7% in urine samples by using PCR and nPCR or real-time PCR respectively. HPV-16 was the predominance HPV type in both sample (10%) followed by HPV-31(6.6%) in both samples and HPV- types 51 and 18 (3.3%) in both samples. There was 3.3% co infection (infection by more than one type of HPV) in placenta and urine samples by types 16 and 51. There was poor agreement of placenta and urine samples results in generic and a moderate agreement for type- specific detection of HPV. The results also showed a significantly difference between number of miscarriage in HPV positive (3.38±0.744) and HPV negative (2.95±0.385) placenta samples. In conclusion HPV infection of trophoblast may cause placental dysfunction and is associated with adverse pregnancy outcomes, including recurrent early pregnancy loss
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