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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 596-600
DOI: https://doi.org/10.20546/ijcmas.2018.703.070


Study to Enumerate the Alterations in Gut Bacterial Population of Eudrilus eugeniae Exposed to Sodium Arsenate
Shefali*, R.K. Gupta and Dharambir Singh
Department of Zoology, CCS Haryana Agricultural University, Hisar-125004, Haryana, India
*Corresponding author
Abstract:

The present study was carried out to envisage the impact of heavy metal i.e. arsenic on the gut bacterial population of epigeic earthworm species, E. eugeniae. For this purpose, three different doses (1.04, 2.09 and 3.13% w/v) of sodium arsenate (Na2HAsO47H2O) were used to treat adult earthworms. The gut samples were serially diluted and inoculated in seven distinct media viz. Jensen's media, King’s medium B base, Burk’s media, Yeast Mannitol Agar media, Vogel- Johnson agar base media, Luria Bertani Broth Miller and Nutrient agar media. Dose dependent decrease in bacterial population was observed in treated worms when compared with control. Significant decrease 64.01%, 43.98%, 21.53%, 37.50%, 44.52%, 33.91% and 26.70% was observed in gut samples isolated from earthworm’s treated with T4 i.e., As(V) (3.13%) as compared to control cultured on Jensen's media, King’s medium B base, Burk’s media, Yeast Mannitol Agar media, Vogel- Johnson agar base media, Luria Bertani Broth Miller and Nutrient agar media respectively. Arsenic poses adverse effect on the health of earthworm as well as alters the microbial population present in gut of earthworms.


Keywords: Earthworm, Media, Arsenate
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How to cite this article:

Shefali, R.K. Gupta and Dharambir Singh. 2018. Study to Enumerate the Alterations in Gut Bacterial Population of Eudrilus eugeniae Exposed to Sodium Arsenate.Int.J.Curr.Microbiol.App.Sci. 7(3): 596-600. doi: https://doi.org/10.20546/ijcmas.2018.703.070