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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 570-582
DOI: https://doi.org/10.20546/ijcmas.2018.703.068


Study of Genetic Diversity in Upland Cotton (Gossypium hirsutum L.) of Cotton Leaf Curl Disease Resistant and Susceptible Genotypes by Using ISSRS
Sonika* and R.S. Sangwan
Department of Genetics and Plant Breeding, CCS, Haryana Agricultural University, Hisar-125004, Haryana, India
*Corresponding author
Abstract:

Cotton is an important fiber cash crop of India and cotton leaf curl disease is the major biotic constraint that can significantly reduce the production and productivity of the crop. Gossypium hirsutum L. suffered losses in Northern part of India mainly in Haryana due to high incidence of cotton leaf curl disease (CLCuD) and “whitefly” which is the vector of this disease. Development of resistant variety to this disease is most effective, long term and safe method to tackle with this problem. First step in this direction is screening and identification of resistant sources and their incorporation in the agronomical superior genotypes/varieties. For this purpose, Genetic diversity between selected resistant (GCH 3 and H 1353) and susceptible (HS 6 and RST 9) parents to cotton leaf curl disease was studied in non-segregating generations i.e. P1, P2 and F1 generations of four G. hirsutum crosses. Twenty eight ISSR primers were used to generate DNA profile of parental genotypes and their F1s with a view to study polymorphism/ genetic diversity. Out of twenty eight ISSR primers, twenty one primers were found as polymorphic. A total of 175 alleles were amplified unambiguously by these 28 ISSR primers, of which 127 alleles were polymorphic (72.57 per cent) and ranged in size from 150-1000 bp. Inspite of per cent polymorphism, the primers showed remarkable polymorphic information content (PIC) values. The PIC value was found in the range of 0.495 to 0.907. The ISSR primer UBC 834 was found to have maximum PIC value (0.907) and was found as more informative to be used in the early screening of the germplasm lines.


Keywords: Agarose gel electrophoresis, Genetic diversity, Genotypes, ISSR primers, Polymorphism
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How to cite this article:

Sonika and Sangwan, R.S. 2018. Study of Genetic Diversity in Upland Cotton (Gossypium hirsutum L.) of Cotton Leaf Curl Disease Resistant and Susceptible Genotypes by Using ISSRS.Int.J.Curr.Microbiol.App.Sci. 7(3): 570-582. doi: https://doi.org/10.20546/ijcmas.2018.703.068