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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 553-557
DOI: https://doi.org/10.20546/ijcmas.2018.703.066


Isolation of Feline Adipose Tissue Derived Mesenchymal Stem Cells
Shazia Nissar1*, Geetha Ramesh2, Sabiha Hayath Basha3,C. Balachandran4 and V. Leela5
1Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, India
2Department of Veterinary Anatomy
3Centre for Stem Cell Research and Regenerative Medicine
4Department of Veterinary Pathology
5Department of Veterinary Physiology, Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, India
*Corresponding author
Abstract:

The omental adipose tissue was collected from, the animals undergone ovariohysterectomy surgical procedures. The samples were digested with 0.1 per cent (W/V) collagenase type I and transferred to a beaker with magnetic stirrer at 600 rpm at 37oC for 30 minutes. The lesser time was given for enzymatic digestion as compared to other species. The viability of the cell was evaluated by trypan blue exclusion method using haematocytometer. Trypan blue had a high affinity to nuclear DNA, which traverse the membrane in a dead cell and dye it blue. The isolated cells were seeded at a density of 1x106 cells per T25 culture flask and incubated in 5 ml of DMEM-HG at 37oC with 5 per cent CO2. 70-80 per cent confluency was observed on day six and 100 percent confluency on day seven.


Keywords: Stem cell, Adipose tissue, Feline, Isolation and Culture
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How to cite this article:

Shazia Nissar, Geetha Ramesh, Sabiha Hayath Basha, C. Balachandran and Leela, V. 2018. Isolation of Feline Adipose Tissue Derived Mesenchymal Stem Cells.Int.J.Curr.Microbiol.App.Sci. 7(3): 553-557. doi: https://doi.org/10.20546/ijcmas.2018.703.066