Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 558-564
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.061


Comparison of Modified Papanicolaou and Hematoxylin and Eosin Stain in Demonstration of Keratin Pearl and Individual Cell Keratin in Oral Squamous Cell Carcinoma
Santhosh Kumar Caliaperoumal1*, R. Vezhavendhan2, PriyaVendhan3 and Uma devi2
1Department of Dentistry, Vinayaka Mission’s Medical college and Hospital, Karaikal, Puducherry, India
2Department of Oral Pathology, Indira Gandhi Dental College and Hospital, Puducherry, India
3Department of Orthodontia, Indira Gandhi Dental College and Hospital, Puducherry, India
*Corresponding author
Abstract:

Keratin pearl (KP) and individual cell keratin(ICK) observation is one of the criteria in grading oral squamous cell carcinoma(SCC). The aim of the study was to evaluate and compare the distinct staining and identification of KP and ICK by routine Hematoxylin & Eosin (H&E) stain and Modified Papanicolaou (PAP) Stain. A total number of 38 paraffin embedded tissues of known cases of well-differentiated and moderately-differentiated SCC were taken and 2 sections of 4-5 micron thickness from each block were cut and stained with H&E stain and modified PAP stain. The KP and ICK were distinctly and clearly stained by modified PAP compared to H&E stain. The positive staining of KP and ICK by modified PAP was statistically significant with P=0.001, f=0.001 and P=0.022, f=0.034 than H&E stain. Based on our findings we conclude that the efficacy of distinct identification of KP and ICK in oral SCC by modified PAP stain is better than H&E stain. So it can be used as an adjuvant in case of oral SCC.


Keywords: Squamous cell carcinoma (SCC), Keratin Pearl(KP), Individual cell Keratin (ICK), modified Papanicolou (PAP) stain, Hematoxylin and Eosin (H&E) stain, differentiation, prognosis.
Download this article as Download

How to cite this article:

Santhosh Kumar Caliaperoumal, R. Vezhavendhan, PriyaVendhan and Uma devi. 2016. Comparison of Modified Papanicolaou and Hematoxylin and Eosin Stain in Demonstration of Keratin Pearl and Individual Cell Keratin in Oral Squamous Cell Carcinoma.Int.J.Curr.Microbiol.App.Sci. 5(7): 558-564. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.061