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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Online ISSN : 2319-7706
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 426-434
DOI: https://doi.org/10.20546/ijcmas.2018.703.050


Molecular Characterization of Heterotic Groups of Cotton through SSR Markers
H.G. Kencharaddi*, R.R. Hanchinal, S.S. Patil, S.M. Manjula, Ravi Kulkarni, C.V. Usharani and C.D. Soregoan
Department of Genetic and Plant Breeding, University of Agricultural Sciences, Dharwad - 580 005, Karnataka, India
*Corresponding author
Abstract:

Knowledge of genetic diversity and relationships among breeding materials has a significant impact on crop improvement. Association between parental divergence and progeny performance has not been well documented in cotton (Gossypium hirsutum L.). This study was conducted to estimate genetic diversity based on simple sequence repeat (SSR) markers among the selected elite cotton genotypes belong to different heterotic groups developed at ARS, Dharwad. A total of 81 alleles were detected from 24 SSR primers. Among these, 62 were polymorphic with an average of 70.29 per cent polymorphism. The number of alleles per SSR locus ranged from 1 to 13 with an average of 3.37. The per cent polymorphism was 0.00 to 100.00 per cent with an average 70.29. Primers viz., BNL 2920, BNL2882, BNL 1059, BNL3171, BNL3994, CIR246, CIR351, CIR070,CIR 100, CIR182, CIR200, CIR238,CIR034, CIR004, CIR373, CIR393, JESPR195 66, JESPR 29 JESPR 58 and NAU3485 exhibited the highest (more than 50%) per cent polymorphism. The number of bands ranged from four (primers BNL 3418) to 91 (primers JESPR58) with an average of 20.40 bands per primer. In case of compact groups, lines DC-ANJ and DC095-7 revealed highest (0.91) similarity coefficient value followed by DC095-7 and DC 4-11 (0.74), DC-ANJ and DC095-7 (0.73). Among robust lines, DRAC 9565 and DR 2 recorded highest (0.78) followed by DRL-88 and DRAC -9565 (0.74). In case stay green groups, highest similarity coefficient value (0.81) was observed between DSG-79-61-1 and DSG-3-5 line followed by DSG-102 and DSG-3-5 (0.79). Among the elite lines of high RGR, highest similarity was observed between DRGR-24-178 and DRGR-257 (0.75) followed by DRGR-32-100 and DRGR-257 (0.71). All the parental lines showed diversity among themselves indicating that there is considerable amount of variation, which can be exploited through appropriate breeding programme.


Keywords: Cotton (Gossypium hirsutum L.), SSR Makers
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How to cite this article:

Kencharaddi, H.G., R.R. Hanchinal, S.S. Patil, S.M. Manjula, Ravi Kulkarni, C.V. Usharani and Soregoan, C.D. 2018. Molecular Characterization of Heterotic Groups of Cotton through SSR Markers.Int.J.Curr.Microbiol.App.Sci. 7(3): 426-434. doi: https://doi.org/10.20546/ijcmas.2018.703.050