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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 404-409
DOI: https://doi.org/10.20546/ijcmas.2018.703.047


Prevalence of Nepoviruses in Strawberry and their Serological Detection
Abhilasha Sharma1*, Anil Handa1, Bunty Shylla2, K.K. Pramanick3, Shelly Kapoor1 and Shalini Verma1
1Department of Plant Pathology, YSPUHF, Nauni, Solan-173230 (H.P), India
2Krishi Vigyan Kendra Kandaghat-173215 (H.P), India
3IARI Regional Station, Shimla-171004 (H.P), India
*Corresponding author
Abstract:

Viruses pose a serious threat to strawberry cultivation all over the world as infections resulting from viruses and related pathogens are limiting factors in the production of certified planting material in strawberry. In Himachal Pradesh, strawberry is fast emerging as a short duration fruit crop yielding lucrative returns. Viruses, however, have emerged as a major hindrance in its commercial cultivation. Direct antigen coating (DAC)-ELISA was performed in major commercial cultivars of strawberry to assess the presence of nematode transmitted nepoviruses in these cultivars as the infected plants developed typical ringspots on leaves, the most striking symptom of nepoviruses. DAC-ELISA resulted in the positive reaction against antibodies of nepoviruses thereby confirming the possible association of nepoviruses in the symptomatic plants. Further, double antibody sandwich (DAS)-ELISA was performed to identify the specific nepoviruses associated with infected strawberry plants. Results obtained in DAS-ELISA established the positive association in the three nepoviruses namely Strawberry Latent Ringspot Virus (SLRSV), Tobacco Ringspot Virus (TRSV) and Raspberry Ringspot Virus (RRSV) in the infected plants. These studies will help in the production of virus indexed planting material of strawberry for developing a sound certification programme in this commercially important crop.


Keywords: ELISA, Strawberry, Nepoviruses
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How to cite this article:

Abhilasha Sharma, Anil Handa, Bunty Shylla, K.K. Pramanick, Shelly Kapoor and Shalini Verma. 2018. Prevalence of Nepoviruses in Strawberry and their Serological Detection.Int.J.Curr.Microbiol.App.Sci. 7(3): 404-409. doi: https://doi.org/10.20546/ijcmas.2018.703.047