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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 292-298
DOI: https://doi.org/10.20546/ijcmas.2018.703.034


Molecular Characterization of Alternaria alternata Causing Fruit Rot of Chilli through RAPD Marker
Kajal Jankar1, Ekta Bagde1, Someshree Mane1, Divya Jambhure2 and Ashwini Kumar3*
1Department of Plant Pathology, Post graduate Institute, Akola-444104, India
2Department of Plant Pathology, Dr. BSKKV, Dapoli- 415712, India
3Department of Plant Pathology, JNKVV, Jabalpur-482001, India
*Corresponding author
Abstract:

Molecular variation in chilli fruit rot pathogen (Alternata alternata) was analyzed by using six isolates collected from major chilli growing region of Maharashtra. The genomic DNA extracted from each isolate of A. alternata was subjected to polymerase chain reaction using 15 primers of OPA and OPB series off which 10 primers produced 83 scorable bands with size ranging from Among the RAPD primer 83 bands 80 bands were polymorphic and level of polymorphism was 96.38%. Molecular diversity using RAPD marker showed that the Aa2 (Satara) having higher similarity index with Aa4 (Akola). Dendrogram generated by pooled molecular data of 10 RAPD primers formed two clusters namely ‘A’ and ‘B’.cluster A include Aa1 and cluster B include Aa2, Aa4, Aa3, Aa5 and Aa6. Thus, the molecular characterization of eight isolates of A. alternate by RAPD revealed existence of variations.


Keywords: Random Amplification of Polymorphic DNA, Molecular characterization, Alternaria alternata, Chilli, fruit rot
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How to cite this article:

Kajal Jankar, Ekta Bagde, Someshree Mane, Divya Jambhure and Ashwini Kumar. 2018. Molecular Characterization of Alternaria alternata Causing Fruit Rot of Chilli through RAPD Marker.Int.J.Curr.Microbiol.App.Sci. 7(3): 292-298. doi: https://doi.org/10.20546/ijcmas.2018.703.034