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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 112-117
DOI: https://doi.org/10.20546/ijcmas.2018.703.012


Screening of Extractable Nuclear Antibodies by ELISA in Patients with Connective Tissue Disorders in a Tertiary Care Hospital
S. Punitha Ranjitham*, I.M. Rejitha, G. Sucilathangam and C. Revathy
Department of Microbiology, Tirunelveli Medical College, Tirunelveli - 627 011, Tamil Nadu, India
*Corresponding author
Abstract:

Autoimmune diseases occur in 3–5% of the population as a result of myriad of genetic and environmental factors that lead to altered immune reactivity. The alterations in the immune system initiated by a loss of immunological tolerance to self-antigens lead to the development of autoreactive phenomena that can be detected in the peripheral blood. The present study was undertaken to detect the Extractable Nuclear Antibodies present in the study population. This study was conducted for a period of 1 year in 100 patients attending Rheumatology department. 50 patients had signs and symptoms related to connective tissue disorders and were ANA positive. Another 50 patients had no signs and symptoms related to CTD and were ANA negative. Out of 100 patients tested, 28 (56%) of ANA positive patients and 3 (6 %) among control group were found to be positive for extractable nuclear antibodies by ENA screening ELISA. Among ENA positive subjects most common age group was 30-39 years that is 38.7% were in this age group. Extractable Nuclear Antibodies were found most predominantly in females (28 out of 31) 90.3% compared to males (3out of 31) 9.7%. Autoimmune connective tissue diseases are rare group of diseases with significant impact on patient’s quality of life and socioeconomic aspects. Antibodies against extractable nuclear antigens (ENAs) are useful diagnostic markers for various autoimmune connective tissue diseases.


Keywords: Autoimmune diseases, Extractable Nuclear Antibodies, ELISA, ANA
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How to cite this article:

Punitha Ranjitham, S., I.M. Rejitha, G. Sucilathangam and Revathy, C. 2018. Screening of Extractable Nuclear Antibodies by ELISA in Patients with Connective Tissue Disorders in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 7(3): 112-117. doi: https://doi.org/10.20546/ijcmas.2018.703.012