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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 3652-3656
DOI: https://doi.org/10.20546/ijcmas.2018.702.434


Comparison of NS-1 Antigen Detection by ICT and ELISA for Evaluating Acute Dengue
Mahesh Kumar and S. Sheethal*
Department of Microbiology, Karnataka Institute of Medical Sciences, KIMS campus, Vidyanagar, Hubballi- 580022, Karnataka, India
*Corresponding author
Abstract:

Dengue is an important mosquito-borne viral disease of humans. Rapid and easy diagnosis of dengue can assist patient triage and care-management. Serological detection of dengue-specific IgM has been the main stay of diagnosis. The detection of dengue NS1 offers a faster presumptive diagnosis. (1) To compare the rapid immunochromatographic card and ELISA methods of NS1 antigen detection. (2) To detect dengue-specific IgM antibodies by MAC-ELISA. Blood specimens collected from patients having febrile illness clinically diagnosed as having dengue fever as per WHO criteria were subjected to detection of NS1 antigen by rapid ICT cards and ELISA, and IgM antibody by MAC-ELISA. Among 116 suspected dengue cases, 25% were positive by NS1 ICT, 29.3% were positive by NS1 ELISA and 37.9% were positive by IgM MAC-ELISA. The sensitivity of NS1 ICT was 52.27% and specificity was 91.66% whereas sensitivity for ELISA was 56.81% and specificity was 87.5%. There was no statistical significance between duration of illness and application of the tests. Overall prevalence of dengue infection was 45.7%. NS1 ELISA showed a slightly better sensitivity but specificity of NS1 ICT was higher. However, for early diagnosis and management of acute dengue, both NS1 antigen and IgM antibody detection tests need to be used in conjunction.


Keywords: Dengue fever, NS1 ICT, NS1 ELISA, IgM MAC-ELISA
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How to cite this article:

Mahesh Kumar, S. and Sheethal, S. 2018. Comparison of NS-1 Antigen Detection by ICT and ELISA for Evaluating Acute Dengue.Int.J.Curr.Microbiol.App.Sci. 7(2): 3652-3656. doi: https://doi.org/10.20546/ijcmas.2018.702.434