National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Dengue is an important mosquito-borne viral disease of humans. Rapid and easy diagnosis of dengue can assist patient triage and care-management. Serological detection of dengue-specific IgM has been the main stay of diagnosis. The detection of dengue NS1 offers a faster presumptive diagnosis. (1) To compare the rapid immunochromatographic card and ELISA methods of NS1 antigen detection. (2) To detect dengue-specific IgM antibodies by MAC-ELISA. Blood specimens collected from patients having febrile illness clinically diagnosed as having dengue fever as per WHO criteria were subjected to detection of NS1 antigen by rapid ICT cards and ELISA, and IgM antibody by MAC-ELISA. Among 116 suspected dengue cases, 25% were positive by NS1 ICT, 29.3% were positive by NS1 ELISA and 37.9% were positive by IgM MAC-ELISA. The sensitivity of NS1 ICT was 52.27% and specificity was 91.66% whereas sensitivity for ELISA was 56.81% and specificity was 87.5%. There was no statistical significance between duration of illness and application of the tests. Overall prevalence of dengue infection was 45.7%. NS1 ELISA showed a slightly better sensitivity but specificity of NS1 ICT was higher. However, for early diagnosis and management of acute dengue, both NS1 antigen and IgM antibody detection tests need to be used in conjunction.
Citations |
 |
 |
 |
 |
