National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The objective of the present study was to characterize an intracellular β-galactosidase from Enterobacter cloacae St. SJ 6. The yield of the enzyme obtained by disrupting the cells using ultrasonication is found to be better compared with other methods. Maximum production of the enzyme is noted in the medium composed of (g/L): Lactose, 15; KH2PO4, 1; KCl, 0.5; NaNO3, 2; MgSO4, 7H2O, 0.5; FeSO4, 7H2O, 0.01; Malt extract, 1; Yeast extract, 1; Agar 2% , pH 7.0±0.2; using 1.5% cell suspension on 72 h cell growth at 30oC.Tryptone enhanced the production enzyme. The obtained Km and Vmax values were 7.92 mM and 134.3µMole/min/mg, respectively. The enzyme has been purified 12.48 fold by Sephadex G-100 chromatography. The optimum pH and temperature for maximum activity of the purified enzyme are found to be 9.0 and 50oC, respectively. Ca2+, Co2+, mercaptoethanol, polyethyleneglycol, sodium azide and CTAB stimulated the activity of the enzyme.
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