Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 492-502
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.054


β-Galactosidase from Enterobacter cloacae: Production, Characterization and Purification
Anamika Ghatak*
Department of Microbiology, Tara Devi Harakhcnand Kankaria Jain College,Cossipore, Kolkata – 700002, India     
*Corresponding author
Abstract:

The objective of the present study was to characterize an intracellular β-galactosidase from Enterobacter cloacae St. SJ 6. The yield of the enzyme obtained by disrupting the cells using ultrasonication is found to be better compared with other methods. Maximum production of the enzyme is noted in the medium composed of (g/L): Lactose, 15; KH2PO4, 1; KCl, 0.5; NaNO3, 2; MgSO4, 7H2O, 0.5; FeSO4, 7H2O, 0.01; Malt extract, 1; Yeast extract, 1; Agar 2% , pH 7.0±0.2; using 1.5% cell suspension on 72 h cell growth at 30oC.Tryptone enhanced the production enzyme. The obtained Km and Vmax values were 7.92 mM and 134.3µMole/min/mg, respectively. The enzyme has been purified 12.48 fold by Sephadex G-100 chromatography. The optimum pH and temperature for maximum activity of the purified enzyme are found to be 9.0 and 50oC, respectively. Ca2+, Co2+, mercaptoethanol, polyethyleneglycol, sodium azide and CTAB stimulated the activity of the enzyme.


Keywords: Enterobacter cloacae, intracellular enzyme, ultrasonication, purification, characterization.
Download this article as Download

How to cite this article:

Anamika Ghatak. 2016. β-Galactosidase from Enterobacter cloacae: Production, Characterization and PurificationInt.J.Curr.Microbiol.App.Sci. 5(7): 492-502. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.054