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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 3457-3465
DOI: https://doi.org/10.20546/ijcmas.2018.702.412


Validation of Molecular Markers Linked to Yellow Mosaic Virus Disease Resistance in Diverse Genotypes of Green Gram (Vigna radiata)
M. Kabi1, T.R. Das2*, B. Baisakh1 and D. Swain1
1Department of Plant Breeding and Genetics, OUAT, Bhubaneswar, Odisha-751001, India
2ICAR-Indian Agriculture Research Institute, Regional Station, Pusa, Bihar- 848125, India
*Corresponding author
Abstract:

Marker assisted indirect selection of resistant genotypes using linked markers has been reported as an effective breeding approach for developing yellow mosaic disease resistant cultivars. Seven genotypes of greengram with different genetic makeup showing resistance reaction to yellow mosaic virus (YMV) were selected along with a susceptible one for molecular confirmation with respect to YMV reactions. These were screened with PCR-based technique employing primer pairs to validate efficiency and reliability of identified markers loci (CYR1 and YMV1). Both CYR1 and YMV1 marker showed consistent polymorphism with respect to disease reaction in seven resistant genotypes. CYR1 was produced an allele size of approximately 90 bp. which concluded that seven genotypes (OBGG-2013-8, OBGG-2013-12, OBGG-2013-11, OBGG-2013-16, OBGG-2013-20, OBGG-2013-21 and OBGG-2013-39) have yellow mosaic virus resistance gene and both the markers are efficient and ubiquitous for genotyping of YMV reaction. OBGG 2013-20 was an YMV resistance and high yielding line which can be used as YMV donor or can be released as a variety after further multi-location evaluations.


Keywords: Greengram, YMV, SCRA, RGA, CYR1
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How to cite this article:

Kabi, M., T.R. Das, B. Baisakh and Swain, D. 2018. Validation of Molecular Markers Linked to Yellow Mosaic Virus Disease Resistance in Diverse Genotypes of Green Gram (Vigna radiata).Int.J.Curr.Microbiol.App.Sci. 7(2): 3457-3465. doi: https://doi.org/10.20546/ijcmas.2018.702.412