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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 3432-3439
DOI: https://doi.org/10.20546/ijcmas.2018.702.409


Proteomic Analysis in Wheat to Study the Effect of Heat Stress on Flag Leaf
A.K. Nandha1*, D.R. Mehta2, N.J. Tulsani1, N. Umretiya1 and N. Delvadiya1
1Department of Biotechnology, Junagadh Agriculture University, Junagadh-362001, India
2Department of Genetics and Plant Breeding, Junagadh Agriculture University,
Junagadh-362001, India
*Corresponding author
Abstract:

The analysis of abiotic stress responsiveness in plants is a very crucial way to identify the genes conferring stress tolerance and their use in crop improvement programme. Heat stress is one of the stresses which highly affect the growth, quality character as well as total yield of the crop. In this study, a proteome analysis by 2D electrophoresis was used to identify the characteristic change in two wheat genotypes viz, GW 451 (heat tolerant) and WH 147 (heat susceptible) from flag leaf. Control and treated leaf samples showed the expression of differentially expressed proteins at booting stage in both genotypes of wheat. After the completion of 2DE, expressed protein were analysed as spot formation on gel. The comparison of two different gel as control and treated for each genotypes gave the information regarding molecular weight, pH, volume of that protein etc. A total of 568 spots were detected in both genotypes among which higher molecular weight spots, 102 kDa and 107 kDa were reported in treated leaf samples of GW 451, while WH 147 contains more numbers of less molecular weight proteins. In this study, various proteins were reported with highly versatile amount of molecular weight. Result of proteomics indicated the tolerance level against heat stress. Comparative proteomic study of plants with and without heat stress allows us to obtain various information regarding how plant adopted defence mechanism against heat stress condition.


Keywords: Triticum aestivum L., Tolerant, Susceptible, Proteome
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How to cite this article:

Nandha, A.K., D.R. Mehta, N.J. Tulsani, N. Umretiya and Delvadiya, N. 2018. Proteomic Analysis in Wheat to Study the Effect of Heat Stress on Flag LeafInt.J.Curr.Microbiol.App.Sci. 7(2): 3432-3439. doi: https://doi.org/10.20546/ijcmas.2018.702.409