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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 3410-3418
DOI: https://doi.org/10.20546/ijcmas.2018.702.406


A Study on Optimization of Exopolysaccharides from a Potential Lactobacillus casei KL14 KX774469
Kanika Sharma*, Nivedita Sharma and Ranjana Sharma
Microbiology Research Laboratory, Department of Basic Sciences, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan (India)-173230, HP, India
*Corresponding author
Abstract:

In this study, an excellent exopolysaccharide producing in house isolate i.e. Lactobacillus casei KL14 with accession number KX774469was used to optimize various independent factors i.e. incubation temperature, pH, carbon source, surfactant source and nitrogen concentration for enhanced exopolysaccharide production by One Factor at a Time (OFAT) approach. The best parameters optimized from OFAT for pH, temperature, incubation time, carbon source, nitrogen concentration, surfactant and carbon concentration were 6.50, 35ËšC, 28 h, sucrose, 0.3%, triton-X100 and 2%. Under optimized conditions, an overall increase of 42.10% EPS production was observed with KL14, respectively. Thus, L. casei KL14 can be used in the speedup production of exopolysaccharides in large scale.


Keywords: Exopolysaccharides, Lactobacillus casei, Optimization, OFAT, Parameters
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How to cite this article:

Kanika Sharma, Nivedita Sharma and Ranjana Sharma. 2018 A Study on Optimization of Exopolysaccharides from a Potential Lactobacillus casei KL14 KX774469.Int.J.Curr.Microbiol.App.Sci. 7(2): 3410-3418. doi: https://doi.org/10.20546/ijcmas.2018.702.406