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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 3281-3287
DOI: https://doi.org/10.20546/ijcmas.2018.702.394


Fine Mapping of Xa7, a Dominant Bacterial Blight Resistance Gene in Rice
Mekala Mallikarjun* and Anil S. Kotasthane
Department of Plant Pathology, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya (IGKV), Raipur – 492012, Chhattisgarh, India
*Corresponding author
Abstract:

Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a devastating disease in rice worldwide. The resistance gene Xa7, which provides dominant resistance against the pathogen with avirulence (Avr) gene AvrXa7, has proved to be durably resistant to BB. A set of SSR markers were selected from the ‘‘gramene’’ database based on the Xa7M5 gene initial mapping region on chromosome 6. This marker was used to construct a high-resolution genetic map of the chromosomal region surrounding the Xa7 gene. An F2 mapping population with 1300 highly susceptible individuals derived from a cross between the near isogenic lines (NILs) IRBB65 and Mahamaya were constructed to localize the Xa7 gene. Xa7 was previously located in a region between two markers M1 (2.2 cM) and M3 (0.5 cM) of chromosome 6 (Porter et al., 2003). Recently, Xa7 was further integrated to the region between two proximal markers GDSSR02 and RM20593, an interval of approximate 118.5 kb (Chen et al., 2008). Finally, the Xa7 gene was mapped to a 0.21-cM interval between the markers GDSSR02 and RM20593. The Xa7-linked markers were landed on the reference sequence of cv. Nipponbare through bioinformatics analysis. A contig map corresponding to the Xa7 gene was constructed. Candidate gene analysis of Xa7 revealed that the fourteen genes encode novel domains that have no amino acid sequence similar to other cloned Xa genes.


Keywords: Bacterial blight, Candidate genes, High-resolution mapping, Marker-assisted selection (MAS), Xa7, Xanthomonas oryzae pv. oryzae (Xoo)
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How to cite this article:

Mekala Mallikarjun and Anil S. Kotasthane. 2018. Fine Mapping of Xa7, a Dominant Bacterial Blight Resistance Gene in Rice.Int.J.Curr.Microbiol.App.Sci. 7(2): 3281-3287. doi: https://doi.org/10.20546/ijcmas.2018.702.394