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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 3215-3219
DOI: https://doi.org/10.20546/ijcmas.2018.702.386


Screening of Auto Antibodies using Indirect Immunofluorescence in Auto Immune Disease Patients
Ambuja Sekar, G. Sucilathangam* and C. Revathy
Department of Microbiology, Tirunelveli Medical College, Tirunelveli - 627 011, Tamil Nadu, India
*Corresponding author
Abstract:

The Hallmark of Autoimmune diseases is the pathologic activity of the immune system of an organism directed against its own cells and tissues. The disease is a direct consequence of tissue and/or organ damage as mediated by auto reactive components of the immune system, that is, auto reactive T lymphocytes and/or auto antibodies. For diagnostic purposes, auto antibodies are the most important analytes. Current clinical practice considers Antinuclear Antibody (ANA) testing as a screening test; this has a major impact on laboratory work with a growing volume of analyses that need to be performed rapidly, to maintain good specificity and sensitivity. The present study was undertaken to determine the rate of ANA positivity and their relationship with the various patterns of the positive specimens. In the present study, a total of 558 samples from patients with clinical suspicion of autoimmune diseases were collected during the period of 2016-17 and was tested for ANA by Indirect Immunofluorescence(IIF) with standard procedures. A total of 558 samples were collected and out of which 76(13.6%) were positive for Antinuclear antibodies with the positivity significantly higher in females. Among the positives, most commonly observed pattern was coarse speckled in 27 cases (35.5%). Information about the pattern types and the distribution of the patterns contribute in diagnosis of autoimmune diseases. It is observed that clinical diagnosis has been supported significantly by ANA test according to data of the present study.


Keywords: Auto antibodies, Autoimmune diseases, Antinuclear antibody, Indirect Immunofluorescence (IIF)
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How to cite this article:

Ambuja Sekar, G. Sucilathangam and Revathy, C. 2018. Screening of Auto Antibodies using Indirect Immunofluorescence in Auto Immune Disease Patients.Int.J.Curr.Microbiol.App.Sci. 7(2): 3215-3219. doi: https://doi.org/10.20546/ijcmas.2018.702.386