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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 461-467
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.049


Purification of Actinomycin D from Streptomyces parvulus Isolated from Mangrove Ecosystem of Kerala, India
R.Cibi and A. Jayakumaran Nair*
 Department of Biotechnology, University of Kerala, Karyavattom, Trivandrum, India
*Corresponding author
Abstract:

The present study was conducted to purify and characterize the active compound  produced from Streptomyces parvulus  for evaluating  its cytotoxic potential against  tumor cell line. The  Streptomyces parvulus CBJ1,  isolated from mangrove ecosystem of Poovar  found to be an efficient producer of bioactive compound. The isolate produced yellow color in ISP2 production  media during submerged fermentation. The orange color compound  was  obtained from mycelia extract using thin layer chromatography and purity was checked using reverse phase HPLC. The purified  compound was  identified as actinomycin D on the basis ofultraviolet-visible spectroscopy, mass spectroscopy and by comparison with  published reference compounds. Theantitumor activity of purified compound, actinomycin D analyzed on A549 cell line by MTTassay. The  actinomycin D showed cytotoxicity on  A549cell line depending on the dose and incubation time. It showed 77% cytotoxicity at concentration of compound 1.25µg/ml with IC50 value of 0.52µg/ml.


Keywords: Streptomyces, actinomycin D, HPLC, cytotoxicity,mass spectrum
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How to cite this article:

Cibi, R., and Jayakumaran Nair, A. 2016. Purification of Actinomycin D from Streptomyces parvulus Isolated from Mangrove Ecosystem of Kerala, India.Int.J.Curr.Microbiol.App.Sci. 5(7): 461-467. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.049