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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 442-460
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.048


Production of Poly-β-hydroxybutyric acid (PHB) by Bacillus cereus
Elsayed B. Belal1* and Mona A. Farid2
1Agricultural Botany Department, (Agricultural Microbiology), Faculty of Agriculture, Kafrelsheikh Univ., 33516, Kafr El-Sheikh, Egypt
2Genetics Department, Faculty of Agriculture, Kafrelsheikh Univ., 33516, Kafr El-Sheikh, Egypt
*Corresponding author
Abstract:

One poly- β-hydroxybutyrate producing bacterial from different microbial sources was isolated and characterized for their morphological, biochemical properties. Based on their 16S rDNA, it was identified as Bacillus cereus E6. The bacterial strain was screened for PHB production and compared for the intensity of fluorescence using Nile red staining method. PHB production conditions were optimized with different carbon and nitrogen sources, the highest PHB production was observed with sucrose and ammonium sulphate by B. cereus, respectively. Regarding incubation time as well as temperature and pH, optimum PHB production conditions were 48 h, 30 °C and 7, respectively. B. cereus is capable of accumulating appreciable levels of PHB from glucose, xylose, lactose, whey, molasses, sugar cane bagasse, rice straw hydroysate when 2% from all substrates were used an alternative carbon for the PHB production. Ammonium sulphate was the best nitrogen source. C/N ratio was also one of the factors that affected the production of PHB. The ratio of C/N that reaches 20:1 was considered the best ratio to produce the highest production of PHB. The present study provide the useful data about the optimized conditions for PHB production by B. cereus that can be utilized for industrial production of PHB, a fast emerging alternative of non-biodegradable plastics.


Keywords: poly-β-hydroxybutyric acid,PHB,Bacillus cereus
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How to cite this article:

Elsayed B. Belal and Mona A. Farid. 2016. Production of Poly-β-hydroxybutyric acid (PHB) by Bacillus cereus.Int.J.Curr.Microbiol.App.Sci. 5(7): 442-460. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.048